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Titolo:
Enhancement of angiotensinogen expression in angiotensin II-dependent hypertension
Autore:
Kobori, H; Harrison-Bernard, LM; Navar, LG;
Indirizzi:
Tulane Univ, Sch Med, Dept Physiol, New Orleans, LA 70112 USA Tulane UnivNew Orleans LA USA 70112 t Physiol, New Orleans, LA 70112 USA
Titolo Testata:
HYPERTENSION
fascicolo: 5, volume: 37, anno: 2001,
pagine: 1329 - 1335
SICI:
0194-911X(200105)37:5<1329:EOAEIA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-PRESSURE REGULATION; RECEPTOR MESSENGER-RNA; TRANSGENIC MICE; INFUSED RATS; KIDNEY; GENE; RENIN; AUGMENTATION; FEEDBACK; PROTEIN;
Keywords:
angiotensin II; angiotensinogen; kidney; sodium, dietary; Western blot; reverse transcriptase-polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Kobori, H Tulane Univ, Sch Med, Dept Physiol, 1430 Tulane Ave, New Orleans, LA 70112USA Tulane Univ 1430 Tulane Ave New Orleans LA USA 70112 LA 70112USA
Citazione:
H. Kobori et al., "Enhancement of angiotensinogen expression in angiotensin II-dependent hypertension", HYPERTENSIO, 37(5), 2001, pp. 1329-1335

Abstract

Chronic infusion of angiotensin (Ang) II leads to the development of hypertension and enhances intrarenal Ang II content to levels greater than can be explained from the circulating concentrations of the peptide. We previously reported that renal angiotensinogen (Ao) mRNA is enhanced in Ang II-dependent hypertension and may contribute to augmented intrarenal Ang II levels, but the Ao protein levels were not significantly increased. Because a high-salt diet (WS) has been shown to suppress renal expression of Ao mRNA, weexamined the effects of chronic Ang II infusion on kidney and liver Ao mRNA and protein levels in male Sprague-Dawley rats (n=12) maintained on an 8%salt diet. Ang II was administered via osmotic minipumps (40 ng/min) to 1 group (n=6) while the remaining rats were sham-operated. A H/S diet alone did not alter systolic blood pressure in sam animals (109 +/-6 mm Hg at day 12); however, Ang II infusions to the WS rats significantly increased systolic blood pressure (167 +/-7 at day 12) and intrarenal Ang II content (459 /- 107 fmoI/g versus 270 +/- 42) despite a marked suppression of plasma renin activity (0.9 +/-0.2 ng Ang I.mL(-1).h(-1) versus 2.8 +/-1.3). Ang II infusions significantly increased kidney Ao mRNA compared with the H/S diet alone by 1.9 +/-0.1-fold. Western blot analysis of kidney protein extracts showed that the Ang II-infused rats had increased kidney Ao protein levels compared with the WS diet alone (1.9 +/-0.1-fold). Liver Ao mRNA and protein and plasma Ao protein were also significantly increased by Ang II infusions. These data demonstrate the effects of Ang II infusion to stimulate Ao mRNA and protein. Thus, the augmented intrarenal Ang II in Ang II-dependent hypertension may result, in part, by a positive amplification mechanism to activate renal expression of Ao.

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Documento generato il 26/01/20 alle ore 10:19:41