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Titolo:
In vivo gene transfer in mouse skeletal muscle mediated by baculovirus vectors
Autore:
Pieroni, L; Maione, D; La Monica, N;
Indirizzi:
IRBM P Angeletti, I-00040 Rome, Italy IRBM P Angeletti Rome Italy I-00040 RBM P Angeletti, I-00040 Rome, Italy
Titolo Testata:
HUMAN GENE THERAPY
fascicolo: 8, volume: 12, anno: 2001,
pagine: 871 - 881
SICI:
1043-0342(20010520)12:8<871:IVGTIM>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOASSOCIATED VIRUS VECTOR; MAMMALIAN-CELLS; INTRAMUSCULAR INJECTION; TRANSGENE EXPRESSION; HUMAN-SERUM; RECOMBINANT BACULOVIRUS; IMMUNOCOMPETENT MICE; COMPLEMENT-SYSTEM; THERAPY; EFFICIENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: La Monica, N IRBM P Angeletti, Via Pontina Km30 600, I-00040 Rome, Italy IRBM P Angeletti Via Pontina Km30 600 Rome Italy I-00040 aly
Citazione:
L. Pieroni et al., "In vivo gene transfer in mouse skeletal muscle mediated by baculovirus vectors", HUM GENE TH, 12(8), 2001, pp. 871-881

Abstract

Baculovirus vectors are efficient tools for gene transfer into mammalian cells in vitro. However, in vitro gene delivery by systemic administration is hindered by the vector inactivation mediated by the complement system. Tocharacterize further the gene transfer efficacy of baculovirus we examinedthe vector transduction efficiency in skeletal muscle. Vectors expressing vesicular stomatitis virus glycoprotein (VSV-G) in the viral envelope were generated by inserting the VSV-G coding sequence downstream of the polyhedrin promoter. Two viruses were constructed to carry either the Escherichia coli beta -galactosidase (beta -Gal) gene or the mouse erythropoietin (EPO) cDNA cloned downstream of the cytomegalovirus immediate-early promoter and enhancer, The greater gene transduction efficiency of the Bac-G-beta Gal vector was confirmed by comparing the betaP-Gal expression level in a varietyof human and mouse cell lines with that obtained on infection with Bac-beta Gal, a vector that lacks VSV-G. Similarly, a 5- to 10-fold increase in beta -Gal expression between Bac-G-beta Gal and Bac-beta Gal was observed when mouse myoblasts and myotubes were infected. The same increase in beta -Gal expression was detected on injection of the Bac-G-beta Gal vector in the quadriceps of BALB/c and C57BL/6 mice. In contrast, a 2-fold difference in transduction was observed between these two vectors in DBA/2J mouse strain. Last, expression of EPO cDNA was detected for at least 178 days in DBA/2J mice on Bac-G-EPO injection into the quadriceps whereas EPO expression declined to normal values by 35 days postinfection in BALB/c and C57BL/6 mice. Thus, these results indicate that baculovirus may be considered a useful vector for gene transfer in mouse skeletal muscle and that persistence of expression may depend on the mouse strain used.

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Documento generato il 02/04/20 alle ore 13:01:44