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Titolo:
Using viral species specificity to define a critical protein/RNA interaction surface
Autore:
Coburn, GA; Wiegand, HL; Kang, YB; Ho, DN; Georgiadis, MM; Cullen, BR;
Indirizzi:
Duke Univ, Med Ctr, Howard Hughes Med Inst, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 oward Hughes Med Inst, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Genet, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 , Med Ctr, Dept Genet, Durham, NC 27710 USA Rutgers State Univ, Waksman Inst, Piscataway, NJ 08854 USA Rutgers State Univ Piscataway NJ USA 08854 Inst, Piscataway, NJ 08854 USA Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA Rutgers State Univ Piscataway NJ USA 08854 Chem, Piscataway, NJ 08854 USA
Titolo Testata:
GENES & DEVELOPMENT
fascicolo: 10, volume: 15, anno: 2001,
pagine: 1194 - 1205
SICI:
0890-9369(20010515)15:10<1194:UVSSTD>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; MESSENGER-RNA EXPORT; CONSTITUTIVE TRANSPORT ELEMENT; REV ACTIVATION DOMAIN; NUCLEAR-PORE COMPLEX; CYCLIN T1; HIV-1 TAT; CELL; PATHWAY; CTE;
Keywords:
gene regulation; nuclear export; retrovirus; RNA binding;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Cullen, BR Duke Univ, Med Ctr, Howard Hughes Med Inst, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 s Med Inst, Durham, NC 27710 USA
Citazione:
G.A. Coburn et al., "Using viral species specificity to define a critical protein/RNA interaction surface", GENE DEV, 15(10), 2001, pp. 1194-1205

Abstract

The Tap protein mediates the sequence-specific nuclear export of mRNAs bearing the retroviral constitutive transport element (CTE) and also plays a critical role in the sequence nonspecific export of cellular mRNAs. Previously, we have demonstrated that CTE function displays species specificity, that is, the CTE functions in human but not quail cells. Here, we demonstratethat quail Tap fails to support CTE function because it cannot bind the CTE. However, changing a single residue in quail Tap, glutamine 246, to arginine, the residue found in human Tap, rescues both CTE function and CTE binding. This residue, which is located on the exterior of a recently reported molecular structure of Tap, defines a surface on Tap that is critical for CTE binding. These data emphasize the potential importance of cross-species genetic complementation in the identification and characterization of cellular factors that are critical for different aspects of viral replication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 21:36:09