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Titolo:
Liposomes as possible carriers for lactoferrin in the local treatment of inflammatory diseases
Autore:
Trif, M; Guillen, C; Vaughan, DM; Telfer, JM; Brewer, JM; Roseanu, A; Brock, JH;
Indirizzi:
Inst Biochem, Bucharest 77700, Romania Inst Biochem Bucharest Romania 77700 t Biochem, Bucharest 77700, Romania Univ Glasgow, Western Infirm, Dept Immunol, Glasgow G11 6NT, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland G11 6NT ow G11 6NT, Lanark, Scotland
Titolo Testata:
EXPERIMENTAL BIOLOGY AND MEDICINE
fascicolo: 6, volume: 226, anno: 2001,
pagine: 559 - 564
SICI:
1535-3702(200106)226:6<559:LAPCFL>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PH-SENSITIVE LIPOSOMES; COLLAGEN-INDUCED ARTHRITIS; ANTIGEN-INDUCED ARTHRITIS; CONJUGATED METHOTREXATE; INTRACELLULAR DELIVERY; RAT; IRON; CELLS; MACROPHAGES; TRANSFERRIN;
Keywords:
liposomes; human lactoferrin; rheumatoid arthritis; human synovial fibroblasts; human synovial fluid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Trif, M Inst Biochem, Spl Independentei 296, Bucharest 77700, Romania InstBiochem Spl Independentei 296 Bucharest Romania 77700 omania
Citazione:
M. Trif et al., "Liposomes as possible carriers for lactoferrin in the local treatment of inflammatory diseases", EXP BIOL ME, 226(6), 2001, pp. 559-564

Abstract

Liposomes prepared from naturally occurring biodegradable and nontoxic lipids are good candidates for local delivery of therapeutic agents. Treatmentof arthritis by intra-articular administration of anti-inflammatory drugs encapsulated in liposomes prolongs the residence time of the drug in the joint. We have previously shown that intra-articular injection of human lactoferrin (hLf), a glycoprotein that possesses anti-inflammatory and antimicrobial activities, into mice with collagen-induced arthritis reduces inflammation. We have now investigated the possibility of using liposome-entrapped hLf as a delivery system to prolong hLf retention at sites of local inflammation such as the rheumatoid joint. Entrapment of hLf in negatively chargedliposomes enhanced its accumulation in cultured human synovial fibroblastsfrom rheumatoid arthritis (RA) patients, compared with positively charged formulations or free protein. However, in the presence of synovial fluid, positively charged liposomes with entrapped hLf were more stable than the negatively charged formulations. In vivo experiments in mice with collagen-induced arthritis showed that the positive liposomes were more efficient in prolonging the residence time of hLf in the inflamed joint as compared with other liposomes, Thus, the amount of hLf retained in the joint after 2 hr was 60% of the injected dose in the case of positive liposomes and only 16% for negative pH-sensitive liposomes, The results suggest that entrapment ofhLf in positively charged liposomes may modify its pharmacodynamic profileand be of therapeutic benefit in the treatment of RA and other local inflammatory conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 22:01:22