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Titolo:
Leptin stimulates gonadotropin releasing hormone release from cultured intact hemihypothalami and enzymatically dispersed neurons
Autore:
Woller, M; Tessmer, S; Neff, D; Nguema, AA; Van Roo, B; Waechter-Brulla, D;
Indirizzi:
Univ Wisconsin, Dept Biol Sci, Whitewater, WI 53190 USA Univ Wisconsin Whitewater WI USA 53190 Biol Sci, Whitewater, WI 53190 USA
Titolo Testata:
EXPERIMENTAL BIOLOGY AND MEDICINE
fascicolo: 6, volume: 226, anno: 2001,
pagine: 591 - 596
SICI:
1535-3702(200106)226:6<591:LSGRHR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
LUTEINIZING-HORMONE; OBESE GENE; REPRODUCTIVE AXIS; FEMALE RATS; PULSATILE; SECRETION; SUPPRESSION; RESPONSES; STERILITY; MONKEY;
Keywords:
GnRH; leptin; hypothalamic tissue culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Woller, M Univ Wisconsin, Dept Biol Sci, Whitewater, WI 53190 USA Univ Wisconsin Whitewater WI USA 53190 Whitewater, WI 53190 USA
Citazione:
M. Woller et al., "Leptin stimulates gonadotropin releasing hormone release from cultured intact hemihypothalami and enzymatically dispersed neurons", EXP BIOL ME, 226(6), 2001, pp. 591-596

Abstract

Leptin is a peptide released by adipocytes that has profound effects on central regulation of body metabolism. The present study represents an investigation into leptin effects on hypothalamic control of reproductive function, specifically on GnRH release. Adult male rats (gonadectomized or sham-operated) were used as donors of hypothalamic tissue that was used as intact hemihypothalami or as enzymatically dispersed hemihypothalami in a perifusion culture system. Continuous samples were collected at 10-min intervals for 8 to 10 hr and were assayed to measure temporal changes in GnRH release in response to Various doses of leptin infused into the perifusion chambers. Leptin at the highest dose (10(-8) M) resulted in consistent and significant stimulation of GnRH release. There were no effects of treatment for surgical preparation (gonadectomy versus sham) or tissue preparation (intact versus dispersed hemihypothalami). The results of this study support the hypothesis that leptin plays a direct stimulatory role in the regulation of GnRH release. This study describes an important step in our understanding of the mechanism that connects changes in basal metabolism with reproductive function. These results indicate an intact interneuronal network is unnecessary for these leptin effects, but does not exclude a role for interneuronal networks in this regulatory pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:23:38