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Titolo:
Dopaminergic retinal cell differentiation in culture: modulation by forskolin and dopamine
Autore:
Guimaraes, MZP; Hokoc, JN; Duvoisin, R; Reis, RAM; de Mello, FG;
Indirizzi:
Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Program Neurobiol, BR-21949900 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21949900 BCeiro, Brazil Cornell Univ, Sch Med, Margaret M Dyson Vis Inst, New York, NY 10021 USA Cornell Univ New York NY USA 10021 Dyson Vis Inst, New York, NY 10021 USA
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 10, volume: 13, anno: 2001,
pagine: 1931 - 1937
SICI:
0953-816X(200105)13:10<1931:DRCDIC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE-HYDROXYLASE; CHICK RETINA; VERTEBRATE RETINA; EMBRYO RETINA; NEURONS; RECEPTORS; EXPRESSION; NEUROTRANSMITTERS; ACTIVATION; PROGENITOR;
Keywords:
c-AMP; chick; D2-like receptor; development; tyrosine hydroxylase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Hokoc, JN Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Program Neurobiol, BR-21949900 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21949900 BCil
Citazione:
M.Z.P. Guimaraes et al., "Dopaminergic retinal cell differentiation in culture: modulation by forskolin and dopamine", EUR J NEURO, 13(10), 2001, pp. 1931-1937

Abstract

We examined the effects of dopamine and cAMP on the differentiation of dopaminergic retinal cells in the chick retina, using an in vitro system and tyrosine hydroxylase immunocytochemistry. Tyrosine hydroxylase-positive cells were detected in cultures prepared from embryonic day 10 retinas. These increased in number as a function of time in vitro and by treatment for 4 days with forskolin. Besides causing a 3.4-fold increase in the tyrosine hydroxylase-positive population, forskolin also caused these cells to developedmorphogenetic features of more mature cells. As opposed to forskolin, cultures treated with dopamine exhibited a 55% reduction of the tyrosine hydroxylase-positive cell population, as compared to untreated cultures. Quinpirole was able to mimic the dopamine effect. This dopamine effect could only be blocked by clozapine, whereas raclopride and eticlopride were ineffective. Our results suggest the existence of a narrow window during development when undifferentiated dopaminergic cells are capable of being influenced by specific signals, possibly via cAMP production. The data also indicate thatdopamine may act as a regulatory factor limiting the tyrosine hydroxylase-positive population in the retina.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 01:19:06