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Titolo:
Electrophysiological characterization of laminar synaptic inputs to the olfactory tubercle of the rat studied in vitro: modulation of glutamatergic transmission by cholinergic agents is pathway-specific
Autore:
Owen, GS; Halliwell, JV;
Indirizzi:
Univ Coll London, Royal Free & Univ Coll Med Sch, Dept Physiol, London NW32PF, England Univ Coll London London England NW3 2PF Physiol, London NW32PF, England
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 9, volume: 13, anno: 2001,
pagine: 1767 - 1780
SICI:
0953-816X(200105)13:9<1767:ECOLSI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
VENTRAL STRIATOPALLIDOTHALAMIC PROJECTION; CENTRAL-NERVOUS-SYSTEM; GUINEA-PIG; CORTEX SLICE; BASAL FOREBRAIN; PIRIFORM CORTEX; RECEPTOR AUTORADIOGRAPHY; MUSCARINIC RECEPTORS; INSITU HYBRIDIZATION; NUCLEUS-ACCUMBENS;
Keywords:
acetylcholine; anticholinesterases; clozapine; synaptic transmission; tacrine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Halliwell, JV Univ Coll London, Royal Free & Univ Coll Med Sch, Dept Physiol, Royal FreeCampus,Rowland Hill St, London NW3 2PF, England Univ Coll London Royal Free Campus,Rowland Hill St London England NW3 2PF
Citazione:
G.S. Owen e J.V. Halliwell, "Electrophysiological characterization of laminar synaptic inputs to the olfactory tubercle of the rat studied in vitro: modulation of glutamatergic transmission by cholinergic agents is pathway-specific", EUR J NEURO, 13(9), 2001, pp. 1767-1780

Abstract

We have exploited the complementary arrangement of afferents in a coronal slice (300-400 mum) of the rat olfactory tubercle (OT) maintained in vitro to investigate transmission in two separate synaptic pathways. We recorded extracellular responses within the OT dense cell layer in slices and stimulated either the outermost layer to activate primary olfactory fibres or deeper to activate secondary input. Superficial stimulation produced a synaptic potential with superimposed population spike. This interpretation was based on blockade by calcium removal from the bathing medium and the use of the glutamate antagonist DNQX (10 mum); the spike was found to be selectivelysuppressed by tetrodotoxin applied near the cells. The spike, but not the synaptic wave, was depressed by 12 mm Ca2+ and enhanced by 1 mm Ba2+ in thebathing medium. Deep stimulation to activate association and intrinsic fibres elicited a nerve volley followed by a later response, also blocked by Ca2+ removal or 10 mum DNQX. It was unaffected by high Ca2+ or Ba2+, hence resulting from synaptic and not action current flow. Removal of Mg2+ from the bathing medium revealed an NMDA component of synaptic transmission at both loci that was selectively blocked by D-AP-5. The deep synaptic response, only, was depressed by carbachol IC50 7 mum or muscarine IC50 13 mum. This depression was also induced by AChE inhibitors eserine or tacrine and was antagonized by 1 mum atropine or 5-10 mum clozapine. These results characterize transmission in the OT and demonstrate a role for muscarinic modulationof deeper synapses in the OT that is influenced by psychotherapeutic drugs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:39:51