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Titolo:
Glutamate receptor targeting in the postsynaptic spine involves mechanismsthat are independent of myosin Va
Autore:
Petralia, RS; Wang, YX; Sans, N; Worley, PF; Hammer, JA; Wenthold, RJ;
Indirizzi:
NIDCD, Neurochem Lab, NIH, Bethesda, MD 20892 USA NIDCD Bethesda MD USA 20892 D, Neurochem Lab, NIH, Bethesda, MD 20892 USA Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 urosci, Baltimore, MD 21205 USA NHLBI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA NHLBI Bethesda MD USA 20892 I, Cell Biol Lab, NIH, Bethesda, MD 20892 USA
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 9, volume: 13, anno: 2001,
pagine: 1722 - 1732
SICI:
0953-816X(200105)13:9<1722:GRTITP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; DENDRITIC SPINES; AMPA RECEPTORS; PURKINJE-CELLS; ENDOPLASMIC-RETICULUM; UNCONVENTIONAL MYOSIN; FIBER SYNAPSES; NMDA RECEPTOR; ACTIN; RAT;
Keywords:
cerebellum; delta; AMPA; dilute mouse; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Petralia, RS NIDCD, Neurochem Lab, NIH, 36-5D08,36 Convent Dr MSC 4162, Bethesda, MD 20892 USA NIDCD 36-5D08,36 Convent Dr MSC 4162 Bethesda MD USA 20892 SA
Citazione:
R.S. Petralia et al., "Glutamate receptor targeting in the postsynaptic spine involves mechanismsthat are independent of myosin Va", EUR J NEURO, 13(9), 2001, pp. 1722-1732

Abstract

Targeting of glutamate receptors (GluRs) to synapses involves rapid movement of intracellular receptors. This occurs in forms of synaptic upregulation of receptors, such as long-term potentiation. Thus, many GluRs are retained in a cytoplasmic pool in dendrites, and are transported to synapses for upregulation, presumably via motor proteins such as myosins travelling along cytoskeletal elements that extend up into the spine. In this ultrastructural immunogold study of the cerebellar cortex, we compared synapses betweennormal rats/mice and dilute lethal mutant mice. These mutant mice lack myosin Va, which has been implicated in protein trafficking at synapses. The postsynaptic spine in the cerebellum lacks the inositol trisphosphate receptor (IP3R) -laden reticular tubules that are found in normal mice and rats (Takagishi et al., Neurosci. Lett., 1996, 215, 169). Thus, we tested the hypothesis that myosin Va is necessary for transport of GluRs and associated proteins to spine synapses. We found that these spines retain a normal distribution of (i) GluRs (delta 1/2, GluR2/3 and mGluR1 alpha), (ii) at least one associated MAGUK (membrane-associated guanylate kinase) protein, (iii) Homer (which interacts with mGluR1 alpha and IP3Rs), (iv) the actin cytoskeleton, (v) the reticulum-associated protein BiP, and (vi) the motor-associated protein, dynein light chain. Thus, while myosin Va may maintain the IP3R-laden reticulum in the spine for proper calcium regulation, other mechanisms must be involved in the delivery of GluRs and associated proteins to synapses. Other possible mechanisms include diffusion along the extrasynaptic membrane and delivery via other motors running along the spine's actin cytoskeleton.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:16:18