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Titolo:
Dendritic attenuation of synaptic potentials in the CA1 region of rat hippocampal slices detected with an optical method
Autore:
Inoue, M; Hashimoto, Y; Kudo, Y; Miyakawa, H;
Indirizzi:
Tokyo Univ Pharm & Life Sci, Lab Cellular Neurobiol, Tokyo 1920392, Japan Tokyo Univ Pharm & Life Sci Tokyo Japan 1920392 ol, Tokyo 1920392, Japan
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 9, volume: 13, anno: 2001,
pagine: 1711 - 1721
SICI:
0953-816X(200105)13:9<1711:DAOSPI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOCATION-DEPENDENT VARIABILITY; NEOCORTICAL PYRAMIDAL NEURONS; PASSIVE MEMBRANE-PROPERTIES; VOLTAGE-SENSITIVE DYE; CABLE PROPERTIES; GUINEA-PIG; CHANNELS; CELLS; DISTAL; INPUT;
Keywords:
dendrite; passive membrane properties; simulation; voltage-sensitive dye;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Inoue, M Tokyo Univ Pharm & Life Sci, Lab Cellular Neurobiol, 1432-1 Horinouchi, Tokyo 1920392, Japan Tokyo Univ Pharm & Life Sci 1432-1 Horinouchi Tokyo Japan 1920392
Citazione:
M. Inoue et al., "Dendritic attenuation of synaptic potentials in the CA1 region of rat hippocampal slices detected with an optical method", EUR J NEURO, 13(9), 2001, pp. 1711-1721

Abstract

We directly measured fast excitatory postsynaptic potentials (EPSPs) alongthe dendrites of hippocampal CA1 pyramidal neurons by employing an opticalmethod to study how synaptic potentials spread along the dendrites. Rat hippocampal slices were stained with a fluorescent voltage-sensitive dye JPW1114 and optical signals were monitored with a 16 x 16 photodiode array system. A stimulating electrode was placed either at stratum lacunosum moleculare to activate perforant fibers that make synaptic contacts to the distal apical dendrites or at stratum oriens to induce EPSPs at the basal dendritesof CA1 pyramidal cells. CNQX-sensitive components of the optical signals, which were assumed to be population EPSPs, were isolated. Propagation and attenuation of the CNQX components were successfully observed with the optical method. At the cell body layer, the peak of the CNQX-sensitive componentwas delayed by 17.08 +/- 1.64 ms from the input sites. Additionally we performed a simulation study to estimate the passive membrane parameters of the apical dendrites. Estimated apparent specific internal axial resistance (R-i) following stratum lacunosum moleculare stimulation was 76.0 +/- 4.2 Omega .cm and apparent specific membrane resistance (R-m) was 27.8 +/- 2.1 k Omega .cm(2) (assuming the specific membrane capacitance of dendrites C-m =1.6 muF/cm(2)). These values are comparable to those previously reported. When synaptic inputs were applied at stratum oriens, these apparent passivemembrane parameters were different (high R-i and low R-m), suggesting thatnonuniform dendritic membrane conductance or voltage-dependent conductances which are active near the resting potential may contribute to the measured passive membrane properties.

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Documento generato il 05/04/20 alle ore 19:13:20