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Titolo:
New insights into the activation of o-xylene biodegradation in Pseudomonasstutzeri OX1 by pathway substrates
Autore:
Arenghi, FLG; Barbieri, P; Bertoni, G; de Lorenzo, V;
Indirizzi:
CSIC, Dept Microbial Biotechnol, Ctr Nacl Biotecnol, E-28049 Madrid, SpainCSIC Madrid Spain E-28049 nol, Ctr Nacl Biotecnol, E-28049 Madrid, Spain Univ Milan, Dipartimento Genet & Biol Microorganismi, I-20133 Milan, ItalyUniv Milan Milan Italy I-20133 Biol Microorganismi, I-20133 Milan, Italy
Titolo Testata:
EMBO REPORTS
fascicolo: 5, volume: 2, anno: 2001,
pagine: 409 - 414
SICI:
1469-221X(200105)2:5<409:NIITAO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING-PROTEIN NTRC; SIGMA(54)-DEPENDENT REGULATOR DMPR; ATPASE ACTIVITY; TRANSCRIPTIONAL ACTIVATOR; NUCLEOTIDE-SEQUENCE; FUNCTIONAL DOMAINS; AROMATIC-COMPOUNDS; GENETIC-EVIDENCE; ENHANCER; XYLR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: de Lorenzo, V CSIC, Dept Microbial Biotechnol, Ctr Nacl Biotecnol, Plaza Murillo 2, E-28049 Madrid, Spain CSIC Plaza Murillo 2 Madrid Spain E-28049 049 Madrid, Spain
Citazione:
F.L.G. Arenghi et al., "New insights into the activation of o-xylene biodegradation in Pseudomonasstutzeri OX1 by pathway substrates", EMBO REP, 2(5), 2001, pp. 409-414

Abstract

The regulation of the tou operon of Pseudomonas stutzeri OX1, for degradation of toluene and o-xylene via phenolic intermediates, has been faithfullyreconstructed in vitro with purified proteins. The set-up included the prokaryotic enhancer-binding protein TouR, the sigma (54)-dependent P-ToMO promoter and the sigma (54)-containing RNA polymerase. With this system we prove that direct binding of 2-methylphenol (o-cresol) to TouR is the only regulatory step for activation of P-ToMO in response to aromatic effecters, thereby ruling out the involvement of other factors or a need for protein processing. In addition, we found that while TouR failed entirely to activate P-ToMO in the absence of inducers, the protein had per se a very significant ATPase activity, which was only moderately increased by o-cresol addition. The results presented here support the view that TouR-like proteins are particularly suitable as evolutionary assets to endow recently evolved pathways for the degradation of environmental pollutants with an optimal degree of transcriptional regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 23:46:41