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Titolo:
Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites
Autore:
Suzuki, Y; Taira, H; Tsunoda, T; Mizushima-Sugano, J; Sese, J; Hata, H; Ota, T; Isogai, O; Tanaka, T; Morishita, S; Okubo, K; Sakaki, Y; Nakamura, Y; Suyama, A; Sugano, S;
Indirizzi:
Univ Tokyo, Dept Virol, Minato Ku, Tokyo 1088639, Japan Univ Tokyo TokyoJapan 1088639 pt Virol, Minato Ku, Tokyo 1088639, Japan Univ Tokyo, Ctr Human Genome, Inst Med Sci, Minato Ku, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Med Sci, Minato Ku, Tokyo 1088639, Japan RIKEN, Genome Sci Ctr, Inst Phys & Chem Res, Wako, Saitama 3510106, Japan RIKEN Wako Saitama Japan 3510106 & Chem Res, Wako, Saitama 3510106, Japan NTT, Intelligent Commun Lab, Commun Sci Labs, Kyoto 6190237, Japan NTT Kyoto Japan 6190237 ommun Lab, Commun Sci Labs, Kyoto 6190237, Japan Univ Tokyo, Dept Complex Sci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 plex Sci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo, Engn Grad Sch Frontier Sci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 tier Sci, Bunkyo Ku, Tokyo 1130033, Japan Helix Res Inst, Chiba 2920812, Japan Helix Res Inst Chiba Japan 2920812Helix Res Inst, Chiba 2920812, Japan Osaka Univ, Inst Mol & Cell Biol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 ll Biol, Suita, Osaka 5650871, Japan Univ Tokyo, Dept Life Sci, Meguro Ku, Tokyo 1530041, Japan Univ Tokyo Tokyo Japan 1530041 Life Sci, Meguro Ku, Tokyo 1530041, Japan
Titolo Testata:
EMBO REPORTS
fascicolo: 5, volume: 2, anno: 2001,
pagine: 388 - 393
SICI:
1469-221X(200105)2:5<388:DTIRBF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-II; MESSENGER-RNAS; GENE; DNA; ALIGNMENT; DATABASE; PROTEINS; ENDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki, Y Univ Tokyo, Dept Virol, Minato Ku, 4-6-1 Shirokanedai, Tokyo 1088639, Japan Univ Tokyo 4-6-1 Shirokanedai Tokyo Japan 1088639 088639, Japan
Citazione:
Y. Suzuki et al., "Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites", EMBO REP, 2(5), 2001, pp. 388-393

Abstract

Determination of the mRNA start site is the first step in identifying the promoter region, which is of key importance for transcriptional regulation of gene expression. The 'oligo-capping' method enabled us to introduce a sequence tag to the first base of an mRNA by replacing the cap structure of the mRNA. Using cDNA libraries made from oligo-capped mRNAs, we could identify the transcriptional start site of an individual mRNA just by sequencing the 5'-end of the cDNA. The fine mapping of transcriptional start sites wasperformed for 5880 mRNAs in 276 human genes. Contrary to our expectations,the majority of the genes showed a diverse distribution of transcriptionalstart sites. They were distributed over 61.7 bp with a standard deviation of 19.5. Our finding may reflect the dynamic nature of transcriptional initiation events of human genes in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:49:57