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Titolo:
In situ transcription and splicing in the Balbiani ring 3 gene
Autore:
Wetterberg, I; Zhao, J; Masich, S; Wieslander, L; Skoglund, U;
Indirizzi:
Karolinska Inst, Med Nobel Inst, Dept Cell & Mol Biol, SE-17177 Stockholm,Sweden Karolinska Inst Stockholm Sweden SE-17177 iol, SE-17177 Stockholm,Sweden Stockholm Univ, Dept Mol Biol & Funct Genom, SE-10691 Stockholm, Sweden Stockholm Univ Stockholm Sweden SE-10691 nom, SE-10691 Stockholm, Sweden
Titolo Testata:
EMBO JOURNAL
fascicolo: 10, volume: 20, anno: 2001,
pagine: 2564 - 2574
SICI:
0261-4189(20010515)20:10<2564:ISTASI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; NUCLEAR RIBONUCLEOPROTEIN-PARTICLES; POLYMERASE-II TRANSCRIPTION; C-TERMINAL DOMAIN; NASCENT TRANSCRIPTS; ELECTRON TOMOGRAPHY; U2 SNRNP; VISUALIZATION; ORGANIZATION; PROTEINS;
Keywords:
gene expression; immunoelectron microscopy; pre-mRNA processing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Wieslander, L Karolinska Inst, Med Nobel Inst, Dept Cell & Mol Biol, SE-17177 Stockholm,Sweden Karolinska Inst Stockholm Sweden SE-17177 Stockholm,Sweden
Citazione:
I. Wetterberg et al., "In situ transcription and splicing in the Balbiani ring 3 gene", EMBO J, 20(10), 2001, pp. 2564-2574

Abstract

The Balbiani ring 3 (BR3) gene contains 38 introns, and more than half of them are co-transcriptionally excised. We have determined the in situ structure of the active BR3 gene by electron tomography, Each of the 20-25 nascent transcripts on the gene is present together with splicing factors and the RNA polymerase II in a nascent transcript and splicing complex, here called the NTS complex. The results indicate that extensive changes in overall shape, substructure and molecular mass take place repeatedly within an NTS complex as it moves along the gene. The volume and calculated mass of the NTS complexes show that, maximally, one complete spliceosome is assembled onthe multi-intron transcript at any given time point, The structural data show that the spliceosome is not a structurally well-defined unit in situ and that the C-terminal domain of the elongating RNA polymerase II cannot carry spliceosomal components for all introns in the BR3 transcript. Our data indicate that spliceosomal factors are continuously added to and released from the NTS complexes during transcription elongation.

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Documento generato il 30/11/20 alle ore 07:08:35