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Titolo:
Phospholipase D1: a key factor for the exocytotic machinery in neuroendocrine cells
Autore:
Vitale, N; Caumont, AS; Chasserot-Golaz, S; Du, G; Wu, S; Sciorra, VA; Morris, AJ; Frohman, MA; Bader, MF;
Indirizzi:
CNRS, UPR 2356, F-67084 Strasbourg, France CNRS Strasbourg France F-67084 NRS, UPR 2356, F-67084 Strasbourg, France SUNY Stony Brook, Dept Pharmacol, Med Ctr, Stony Brook, NY 11794 USA SUNY Stony Brook Stony Brook NY USA 11794 Ctr, Stony Brook, NY 11794 USA SUNY Stony Brook, Ctr Dev Genet, Med Ctr, Stony Brook, NY 11794 USA SUNY Stony Brook Stony Brook NY USA 11794 Ctr, Stony Brook, NY 11794 USA
Titolo Testata:
EMBO JOURNAL
fascicolo: 10, volume: 20, anno: 2001,
pagine: 2424 - 2434
SICI:
0261-4189(20010515)20:10<2424:PDAKFF>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; ADP-RIBOSYLATION-FACTOR; ADRENAL CHROMAFFIN CELLS; REGULATED EXOCYTOSIS; NEUROTRANSMITTER RELEASE; MEMBRANE-FUSION; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; LYSOPHOSPHATIDIC ACID; ENHANCES EXOCYTOSIS; SECRETORY GRANULES;
Keywords:
chromaffin cell; cytoskeleton; exocytosis; phospholipase D; secretory granule;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Bader, MF CNRS, UPR 2356, 5 Rue Blaise Pascal, F-67084 Strasbourg, France CNRS 5 Rue Blaise Pascal Strasbourg France F-67084 ourg, France
Citazione:
N. Vitale et al., "Phospholipase D1: a key factor for the exocytotic machinery in neuroendocrine cells", EMBO J, 20(10), 2001, pp. 2424-2434

Abstract

Phospholipase D (PLD) has been proposed to mediate cytoskeletal remodelingand vesicular trafficking along the secretory pathway. We recently described the activation of an ADP ribosylation factor-regulated PLD at the plasmamembrane of chromaffin cells undergoing secretagogue-stimulated exocytosis, We show here that the isoform involved is PLD1b, and, using a real-time assay for individual cells, that PLD activation and exocytosis are closely correlated. Moreover, overexpressed PLD1, but not PLD2, increases stimulatedexocytosis in a phosphatidylinositol 4,5-bisphosphate-dependent manner, whereas catalytically inactive PLD1 inhibits it. These results provide the first direct evidence that PLD1 is an important component of the exocytotic machinery in neuroendocrine cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:43:59