Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Human telomerase reverse transcriptase expression in Diff-Quik-srained FNAsamples from thyroid nodules
Autore:
Siddiqui, MT; Greene, KL; Clark, DP; Xydas, S; Udelsman, R; Smallridge, RC; Zeiger, MA; Saji, M;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 Pathol, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Dept Surg, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 t Surg, Baltimore, MD 21205 USA Mayo Clin Jacksonville, Dept Med, Jacksonville, FL 32224 USA Mayo Clin Jacksonville Jacksonville FL USA 32224 cksonville, FL 32224 USA
Titolo Testata:
DIAGNOSTIC MOLECULAR PATHOLOGY
fascicolo: 2, volume: 10, anno: 2001,
pagine: 123 - 129
SICI:
1052-9551(200106)10:2<123:HTRTEI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
FINE-NEEDLE ASPIRATION; FIBRONECTIN MESSENGER-RNA; POLYMERASE CHAIN-REACTION; HTERT GENE-EXPRESSION; RENAL-CELL CARCINOMA; IMMORTAL HUMAN-CELLS; THYROTROPIN RECEPTOR; MAMMALIAN TELOMERASE; CATALYTIC SUBUNIT; PREOPERATIVE DIAGNOSIS;
Keywords:
molecular marker; thyroid carcinoma; cytopathology; telomerase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Saji, M MedStar Res Inst, Endocrinol Lab, 108 Irving St, Washington, DC 20010 USA MedStar Res Inst 108 Irving St Washington DC USA 20010 C 20010 USA
Citazione:
M.T. Siddiqui et al., "Human telomerase reverse transcriptase expression in Diff-Quik-srained FNAsamples from thyroid nodules", DIAGN MOL P, 10(2), 2001, pp. 123-129

Abstract

Fine-needle aspiration (FNA) is a highly sensitive method in the differential diagnosis of thyroid nodules. However, 10% of thyroid FNAs are indeterminate for cancer, and thus additional markers may be useful diagnostically. The authors have demonstrated previously that human telomerase reverse transcriptase (hTERT) gene expression is useful in the distinction of benign lesions from malignant lesions. They therefore wondered whether the detection of hTERT gene expression was feasible using archival slides. To establishan experimental system, ribonucleic acid was extracted from human anaplastic thyroid carcinoma cell line (ARO) in cytologic specimens, and reverse transcription-polymerase chain reaction (RT-PCR) for hTERT expression was performed. RT-PCR analysis for hTERT gene detection was then performed using 58 Diff-Quik-stained archival FNA samples collected retrospectively. RT-PCR for human thyroglobulin (hTg) or B-actin gene expression served as a positive control. Successful PCR results were obtained from 48 of the 58 cases. All 10 slides in which no RT-PCR products were noted were older than 3 years. hTERT gene expression was demonstrated in FNAs from two of seven cases (29%) of hyperplastic nodule, one of one case (100%) of Hashimoto's thyroiditis, three of eight cases (38%) of follicular adenoma, three of eight cases (38%) of Hurthle cell adenoma, three of four cases (75%) of follicular carcinoma, two of two cases (100%) of Hurthle cell carcinoma, and 11 of 18 cases (61%) of papillary carcinoma. All but one of the available 33 corresponding frozen samples exhibited the same RT-PCR results. This study demonstrates that Diff-Quik-stained thyroid FNA specimens less than 3 years old can beused for the detection of hTERT gene expression by RT-PCR. This test, along with careful cytopathologic examination, may improve our ability to differentiate benign lesions from malignant lesions in indeterminate FNA samplesfrom thyroid nodules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:09:02