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Titolo:
Differential biomarker gene and protein expressions in nonylphenol and estradiol-17 beta treated juvenile rainbow trout (Oncorhynchus mykiss)
Autore:
Arukwe, A; Kullman, SW; Hinton, DE;
Indirizzi:
Univ Bergen, HIB, Dept Biol Mol, N-5020 Bergen, Norway Univ Bergen Bergen Norway N-5020 B, Dept Biol Mol, N-5020 Bergen, Norway Univ Calif Davis, Sch Vet Med, Dept Anat Physiol & Cell Biol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 siol & Cell Biol, Davis, CA 95616 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
fascicolo: 1, volume: 129, anno: 2001,
pagine: 1 - 10
SICI:
1532-0456(200105)129:1<1:DBGAPE>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ESTROGEN-RECEPTOR; ZONA RADIATA PROTEINS; SALMON SALMO-SALAR; IN-VITRO; VITELLOGENIN INDUCTION; ENDOCRINE DISRUPTORS; PHOSPHORYLATION; 4-NONYLPHENOL; XENOBIOTICS; CLONING;
Keywords:
estrogen-receptor; Zr-protein; vitellogenin; biomarkers; gene expression; xenoestrogen; rainbow trout;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Arukwe, A Univ Bergen, HIB, Dept Biol Mol, POB 7800, N-5020 Bergen, NorwayUniv Bergen POB 7800 Bergen Norway N-5020 N-5020 Bergen, Norway
Citazione:
A. Arukwe et al., "Differential biomarker gene and protein expressions in nonylphenol and estradiol-17 beta treated juvenile rainbow trout (Oncorhynchus mykiss)", COMP BIOC C, 129(1), 2001, pp. 1-10

Abstract

The time- and dose-dependent transcriptional and translational expression of biomarker genes in nonylphenol (NP) and estradiol-17 beta (E,) treated juvenile rainbow trout is reported. Fish were exposed to NP (1, 5 and 25 mg/kg) and E-2 (5 mg/kg) and killed at 2, 6, 12, 24, 48 and 72 h after exposure. The estrogen receptor (ER), vitellogenin (Vtg) and eggshell zona radiataprotein (Zr-protein) gene expressions were analyzed in total liver RNA using Northern and slot hybridization with specific cDNA probes. Plasma Vtg and Zr-protein levels were evaluated using indirect ELISA. While ZI-protein gene showed an induction only at 24 h post-exposure, the plasma protein levels showed a time-dependent increase in the 25-mg NP treated group. Vtg transcripts showed an apparent time-dependent increase without a concomitant increase in protein levels in the 25-mg NP treated fish. Time-dependent increases in Vtg and Zr-protein gene expressions without the corresponding increases in ER gene transcription was observed in E-2-treated fish at 2, 6 and 12 h post-exposure. Induction of ER gene transcripts was observed from 24 hand did not change significantly at 48 and 72 h. In the E-2-treated fish, induction of plasma Vtg levels was observed at 48 and 72 h, while plasma Zr-protein was induced at 24, 48 and 72 h, after exposure. We conclude that the E-2- and NP-induced Vtg and Zr-protein gene expressions at the early time intervals after exposure are not dependent on increase in the transcriptional activity of the ER gene and that Vtg and Zr-protein gene transcriptions require only basal or minimal ER concentration, in addition to other mechanisms. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:04:32