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Titolo:
Sodium block and depolarization diminish P2Z-dependent Ca2+ entry in humanB lymphocytes
Autore:
Lohn, M; Klapperstuck, M; Riemann, D; Markwardt, F;
Indirizzi:
Univ Halle Wittenberg, Julius Bernstein Inst Physiol, D-06097 Halle, Germany Univ Halle Wittenberg Halle Germany D-06097 siol, D-06097 Halle, Germany Max Delbruck Ctr Mol Med, Franz Volhard Klin, Berlin, Germany Max DelbruckCtr Mol Med Berlin Germany z Volhard Klin, Berlin, Germany Univ Halle Wittenberg, Inst Med Immunol, D-06097 Halle, Germany Univ HalleWittenberg Halle Germany D-06097 unol, D-06097 Halle, Germany
Titolo Testata:
CELL CALCIUM
fascicolo: 6, volume: 29, anno: 2001,
pagine: 395 - 408
SICI:
0143-4160(200106)29:6<395:SBADDP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT P2X(7) RECEPTOR; OPERATED ION-CHANNEL; EXTRACELLULAR ATP; PURINERGIC RECEPTORS; P2Z PURINOCEPTORS; XENOPUS-OOCYTES; CALCIUM INFLUX; CYTOLYTIC PORE; P-2Z RECEPTOR; L-SELECTIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Klapperstuck, M Univ Halle Wittenberg, Julius Bernstein Inst Physiol, Magdeburger Str 6, D-06097 Halle, Germany Univ Halle Wittenberg Magdeburger Str6 Halle Germany D-06097
Citazione:
M. Lohn et al., "Sodium block and depolarization diminish P2Z-dependent Ca2+ entry in humanB lymphocytes", CELL CALC, 29(6), 2001, pp. 395-408

Abstract

Despite a high Ca2+-permeability of the P2Z receptor in human B lymphocytes, extracellular ATP(4-) has only a minor effect on global [Ca2+](i). The aim of this study was to reveal the mechanisms responsible for this discrepancy. We investigated the relationship between ATP(4-)-application, Ca-i(2+)-response, membrane current and membrane potential in two human B cell lines and in human tonsillar B cells. This Was achieved by a combination of FACS- and voltage clamp measurements and the usage of appropriate voltage- andCa2+-sensitive fluorescent dyes. ATP(4-)-induced changes in whole-cell current and [Ca2+](i) were blocked by extracellular as well as intracellular Naf. Under current clamp conditions, ATP(4-)-induced Nat-entry diminished the Ca2+ entry via reduction of the driving force. A substantial increase in [Ca2+-](i) induced by ATP(4-) was only observed in Na+-free solutions. The pathway of signal transduction activated by ATP(4-) via P2Z receptor of human B lymphocytes under physiological conditions seems not to operate by an increase in the global intracellular Ca2+-concentration, but rather bythe depolarization of the cell membrane as a result of the Na+-influx. (C)2001 Harcourt Publishers Ltd.

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Documento generato il 02/12/20 alle ore 05:32:04