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Titolo:
Determination of carbohydrates as their p-sulfophenylhydrazones by capillary zone electrophoresis
Autore:
Wang, XY; Chen, Y;
Indirizzi:
Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Grp 205, Beijing 100080, PeoplesR China Chinese Acad Sci Beijing Peoples R China 100080 g 100080, PeoplesR China
Titolo Testata:
CARBOHYDRATE RESEARCH
fascicolo: 2, volume: 332, anno: 2001,
pagine: 191 - 196
SICI:
0008-6215(20010518)332:2<191:DOCATP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; REDUCING SUGARS; THIN-LAYER; MONOSACCHARIDES; DERIVATIVES; HYDRAZINE; OLIGOSACCHARIDES; REAGENT; ACIDS;
Keywords:
p-hydrazinobenzenesulfonic acid; carbohydrate; capillary electrophoresis; precolumn derivatization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, Y Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Grp 205, POB 2709, Beijing 100080, Peoples R China Chinese Acad Sci POB 2709 Beijing Peoples R China 100080 R China
Citazione:
X.Y. Wang e Y. Chen, "Determination of carbohydrates as their p-sulfophenylhydrazones by capillary zone electrophoresis", CARBOHY RES, 332(2), 2001, pp. 191-196

Abstract

p-Hydrazinobenzenesulfonic acid was explored as an ultraviolet labeling reagent for capillary electrophoresis of mono-, di- and trisaccharides. The labeling reaction that produces p-sulfophenylhydrazines took less than 8 min, and introduced both chromphore and charged groups into the carbohydrate molecules. The derivatives of nine mono- and disaccharides were completely separated in 9 min using a 100 mM berate buffer at pH 10.24. On-column UV detection at 200 nm allowed the detection of glucose with a mass detection limit of 17.6 fmol or a concentration limit of 3.6 muM. Reproducible quantification of carbohydrates was achieved in the concentration range of 0.1-9.1 mM in reaction solution. The method was applied successfully to determine the monosaccharide composition of laminaran. (C) 2001 Elsevier Science Ltd. All rights reserved.

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Documento generato il 29/03/20 alle ore 18:09:01