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Titolo:
Regulation of tumor angiogenesis by oxygen-regulated protein 150, an inducible endoplasmic reticulum chaperone
Autore:
Ozawa, K; Tsukamoto, Y; Hori, O; Kitao, Y; Yanagi, H; Stern, DM; Ogawa, S;
Indirizzi:
Kanazawa Univ, Dept Neuroanat, Kanazawa, Ishikawa 9208640, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9208640 wa, Ishikawa 9208640, Japan Columbia Univ Coll Phys & Surg, Dept Physiol & Cellular Biophys, New York,NY 10032 USA Columbia Univ Coll Phys & Surg New York NY USA 10032 ew York,NY 10032 USA HSP Res Inst, Kyoto 6150061, Japan HSP Res Inst Kyoto Japan 6150061HSP Res Inst, Kyoto 6150061, Japan Natl Cardiovasc Ctr, Dept Pathol, Osaka, Japan Natl Cardiovasc Ctr OsakaJapan rdiovasc Ctr, Dept Pathol, Osaka, Japan
Titolo Testata:
CANCER RESEARCH
fascicolo: 10, volume: 61, anno: 2001,
pagine: 4206 - 4213
SICI:
0008-5472(20010515)61:10<4206:ROTABO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; VASCULAR-PERMEABILITY FACTOR; IN-VIVO; EXPRESSION; INDUCTION; HYPOXIA; ORP150; CELLS; GENE; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Ozawa, K Kanazawa Univ, Dept Neuroanat, 13-1 Takaramachi, Kanazawa, Ishikawa 9208640, Japan Kanazawa Univ 13-1 Takaramachi Kanazawa Ishikawa Japan 9208640 an
Citazione:
K. Ozawa et al., "Regulation of tumor angiogenesis by oxygen-regulated protein 150, an inducible endoplasmic reticulum chaperone", CANCER RES, 61(10), 2001, pp. 4206-4213

Abstract

Expression of angiogenic factors such as vascular endothelial growth factor (VEGF) under conditions of cell stress involves both transcriptional and translational events, as well as an important role for inducible endoplasmic reticulum (ER) chaperones, Coexpression of VEGF and 150-kDa oxygen-regulated protein (ORP), a novel ER chaperone, in human glioblastoma suggested a link between angiogenesis and ORP150, C6 glioma cells stably transfected with ORP150 antisense displayed selectively reduced ORP150 expression. Tumorsraised after inoculation of immuno-compromised mice with ORP150 antisense C6 glioma transfectants demonstrated an initial phase of growth comparable to wild-type C6 glioma cells which was followed by marked regression within8 days. Decreased density of platelet/endothelial cell adhesion molecule 1-positive structures within the tumor bed was consistent with reduced angiogenesis in C6 gliomas expressing ORP150 antisense, compared with tumors derived from C6 cells overexpressing ORP150 sense or vector controls. In vitro, inhibition of ORP150 expression decreased release of VEGF into culture supernatants; in ORP150 antisense transfectants, VEGF accumulated intracellularly within the ER. These findings demonstrate a critical role for the inducible ER chaperone ORP150 in tumor-mediated angiogenesis via processing of VEGF, and, thus, highlight a neu. facet of angiogenic mechanisms amenable to therapeutic manipulation in tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 04:13:45