Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Construction of peptide conjugates with peptide nucleic acids containing an anthracene probe and their interactions with DNA
Autore:
Balasundaram, G; Takahashi, T; Ueno, A; Mihara, H;
Indirizzi:
Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Bioengn, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol Yokohama Kanagawa Japan 2268501anagawa 2268501, Japan Tokyo Inst Technol, Japan Sci & Technol Corp, PRESTO, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol Yokohama Kanagawa Japan 2268501 anagawa 2268501, Japan
Titolo Testata:
BIOORGANIC & MEDICINAL CHEMISTRY
fascicolo: 5, volume: 9, anno: 2001,
pagine: 1115 - 1121
SICI:
0968-0896(200105)9:5<1115:COPCWP>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE SYNTHESIS; INTERCALATING DRUGS; CIRCULAR-DICHROISM; THYMINE; RECOGNITION; CYTOSINE; ADENINE; GUANINE; BINDING; PNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Mihara, H Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Bioengn, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol Yokohama Kanagawa Japan 2268501 68501, Japan
Citazione:
G. Balasundaram et al., "Construction of peptide conjugates with peptide nucleic acids containing an anthracene probe and their interactions with DNA", BIO MED CH, 9(5), 2001, pp. 1115-1121

Abstract

We designed and synthesized the peptide nucleic acid (PNA)-peptide conjugates having anthracene chromophores and investigated their interactions withcalf thymus DNA, [d(AT)(10)](2), [d(GC)(10)](2), and [d(AT)(10)dA(6)](2). Considering the synthesis compatibility and expecting that a novel DNA analogue, PNA, can improve DNA binding properties of alpha -helix peptides, we attempted to attach thymine PNA oligomers at the C-terminus of a 14 amino acid alpha -helix peptide that contained a pair of artificial intercalators,anthracene, as a probe, and to examine their interactions with DNA using anthracene UV, fluorescence and circular dichroism properties. The results observed in this study showed that the designed peptide folded in an alpha -helix structure in the presence of calf thymus DNA, [d(AT)(10)](2) and [d(AT)(10)dA(6)](2) with the chromophores at the side-chain being fixed with a left-handed chiral-sense orientation. The alpha -helix and the anthracene signals were not observed for [d(GC)(10)](2) Incorporation of thymine PNA oligomers into the designed alpha -helix peptide increased the DNA binding ability to [d(AT)(10)dA(6)](2) with increasing the length of the PNA without changing the conformations of the peptide backbone and the anthracene side-chains. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:05:48