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Titolo:
Phenotype-dependent modulation of elastin and collagen expression by elastin peptide Val-Pro-Gly in chick smooth muscle cells in vitro
Autore:
Hirano, E; Taira, K; Akiyama, M; Tanaka, N; Okamoto, K; Tajima, S;
Indirizzi:
Natl Def Med Coll, Dept Dermatol, Tokorozawa, Saitama 3598513, Japan Natl Def Med Coll Tokorozawa Saitama Japan 3598513 Saitama 3598513, Japan Kyushu Inst Technol, Dept Biochem Engn & Sci, Iizuka, Fukuoka 8200067, Japan Kyushu Inst Technol Iizuka Fukuoka Japan 8200067 , Fukuoka 8200067, Japan
Titolo Testata:
BIOMEDICAL RESEARCH-TOKYO
fascicolo: 1, volume: 22, anno: 2001,
pagine: 25 - 31
SICI:
0388-6107(200102)22:1<25:PMOEAC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPLEMENTARY-DNA; SEQUENCE-ANALYSIS; ACID; TROPOELASTIN; FIBROBLASTS; CLONES; CDNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Tajima, S Natl Def Med Coll, Dept Dermatol, 3-2 Namiki, Tokorozawa, Saitama 3598513,Japan Natl Def Med Coll 3-2 Namiki Tokorozawa Saitama Japan 3598513 an
Citazione:
E. Hirano et al., "Phenotype-dependent modulation of elastin and collagen expression by elastin peptide Val-Pro-Gly in chick smooth muscle cells in vitro", BIOMED RES, 22(1), 2001, pp. 25-31

Abstract

Cultured smooth muscle cells (SMCs) explanted from 20-day-old chick embryoaortas exhibited a spontaneous phenotype modulation from 'contractile' (day 1 in culture) to 'synthetic' (days 3 and 5 in culture) state on the basisof the reduced expressions of calponin and a-actin. The cells on day 1 (contractile state) and day 3 (synthetic state) in culture were treated with chick elastin fragment Val-Pro-Gly (VPC) and responses of cell migration andextracellular matrix (elastin and collagen) expression were compared. Maximum chemotactic activity of SMCs towards VPG was observed at the dose of 10(-7) M in the synthetic phenotype but none in the contractile phenotype. Bycontrast, VPG increased mRNA levels of elastin and type I collagen at the dose of 10(-7) M in the cells on day 1 (contractile state), but rather decreased them in the cells on day 3 (synthetic state). Biosynthesis of elastinand collagen in the cells on day 1 and day 3 essentially paralleled the change of their mRNA levels. The results indicate that the effect of elastin peptide on extracellular matrix expression and chemotactic response is dependent on the phenotypes of SMC. These findings suggest that the different responses of SMC to elastin fragments may play an important role in the fibrotic change of arteriosclerosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 01:33:34