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Titolo:
Spot compliant neuronal networks by structure optimized micro-contact printing
Autore:
Lauer, L; Klein, C; Offenhausser, A;
Indirizzi:
Max Planck Inst Polymer Res, D-55128 Mainz, Germany Max Planck Inst Polymer Res Mainz Germany D-55128 D-55128 Mainz, Germany Univ Mainz, Inst Physiol Chem & Pathobiochem, D-55099 Mainz, Germany Univ Mainz Mainz Germany D-55099 & Pathobiochem, D-55099 Mainz, Germany
Titolo Testata:
BIOMATERIALS
fascicolo: 13, volume: 22, anno: 2001,
pagine: 1925 - 1932
SICI:
0142-9612(200107)22:13<1925:SCNNBS>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; HIPPOCAMPAL-NEURONS; CELL-SHAPE; PATTERNS; TRANSISTOR; POLARITY; SURFACES; CULTURE; LINE;
Keywords:
micro-contact printing (mu CP); neuronal networks; PCC12 MzN neuroblastoma cells; structure engineering; nodal compliance; induced cell differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Offenhausser, A Max Planck Inst Polymer Res, Ackermannweg 10, D-55128 Mainz, Germany Max Planck Inst Polymer Res Ackermannweg 10 Mainz Germany D-55128
Citazione:
L. Lauer et al., "Spot compliant neuronal networks by structure optimized micro-contact printing", BIOMATERIAL, 22(13), 2001, pp. 1925-1932

Abstract

Neuronal cell growth in vitro can be controlled with micropatterned structures of extracellular matrix proteins such as laminin. This technique is a powerful tool for studying neuronal cell function in order to increase experimental reproducibility and to specifically design innovative experimentalsetups. Tn this paper the correlation between the structural dimensions ofthe ECM pattern and the shape of the resulting cellular network is analyzed. The aim of the present study was to position neuronal cell bodies as precisely as possible and to induce directed cell differentiation. PCC7-MzN cells were cultured on laminin patterns. The line width, node size and gap size in-between cell adhesion sites was varied systematically. Micrographs ofthe samples were taken and statistically analyzed using Student's t-test and linear correlation methods. Precise cell positioning has successfully been performed and evidence For controlled neuronal polarization has been found. With a structure geometry of 4 mum line width, 20 mum node size and 10 mum gap size a nodal compliance of 86% (+/- 10%) has been achieved. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:23:09