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Titolo:
Altering intracellular pH disrupts development and cellular organization in preimplantation hamster embryos
Autore:
Squirrell, JM; Lane, M; Bavister, BD;
Indirizzi:
Univ Wisconsin, Mol Biol Lab, Dept Anim Hlth & Biomed Sci, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 h & Biomed Sci, Madison, WI 53706 USA
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 6, volume: 64, anno: 2001,
pagine: 1845 - 1854
SICI:
0006-3363(200106)64:6<1845:AIPDDA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEA-URCHIN EGG; MICROTUBULE-MEDIATED MOTILITY; CYTOPLASMIC PH; 2-CELL EMBRYOS; ACTIVE MITOCHONDRIA; SPISULA-SOLIDISSIMA; NA+/H+ ANTIPORTER; PROTEIN-SYNTHESIS; SPERM MOTILITY; BOVINE SPERM;
Keywords:
developmental biology; early development; embryo; gamete biology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Squirrell, JM Univ Wisconsin, Mol Biol Lab, Dept Anim Hlth & Biomed Sci, 1525 Linden Dr,Madison, WI 53706 USA Univ Wisconsin 1525 Linden Dr Madison WI USA 53706 53706 USA
Citazione:
J.M. Squirrell et al., "Altering intracellular pH disrupts development and cellular organization in preimplantation hamster embryos", BIOL REPROD, 64(6), 2001, pp. 1845-1854

Abstract

In early cleavage stage hamster embryos, the inability to regulate intracellular pH (pH(i)) properly is associated with reduced developmental competence in vitro. The disruption of mitochondrial organization is also correlated with reduced development in vitro. To determine the relationship betweenpH(i) acid the disruption of cytoplasmic organization, we examined the effects of altering pH, on hamster embryo development, mitochondrial distribution, and cytoskeletal organization. The weak base trimethylamine! was used to increase pH(i) and was found to reduce embryo development and disrupt the perinuclear organization of mitochondria. The weak acid 5,5-dimethyl-2,4-oxazolinedione was used to decrease pH(i) and was also found to reduce development and disrupt the perinuclear organization of mitochondria. With either treatment, the microfilament organization was perturbed, but the microtubule cytoskeleton was not. However, the temporal progression of the disruption of mitochondrial distribution was more rapid in alkalinized embryos than acidified embryos, as revealed by two-photon imaging of living embryos. Additionally, the disruption of the microfilament network by the two treatments was not identical. The cytoplasmic disruptions observed were not due toacute toxicity of the compounds because embryos recovered developmentally when the treatment compounds were removed. These observations link ionic homeostasis, structural integrity and developmental competence in preimplantation hamster embryos.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:15:17