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Titolo:
Growth hormone (GH)/GH receptor expression and GH-mediated effects during early bovine embryogenesis
Autore:
Kolle, S; Stojkovic, M; Prelle, K; Waters, M; Wolf, E; Sinowatz, F;
Indirizzi:
Univ Munich, Dept Vet Anat 2, D-80539 Munich, Germany Univ Munich MunichGermany D-80539 t Vet Anat 2, D-80539 Munich, Germany Dept Mol Anim Breeding & Genet, D-81377 Munich, Germany Dept Mol Anim Breeding & Genet Munich Germany D-81377 77 Munich, Germany Univ Queensland, Dept Physiol & Pharmacol, St Lucia, Qld 4072, Australia Univ Queensland St Lucia Qld Australia 4072 St Lucia, Qld 4072, Australia
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 6, volume: 64, anno: 2001,
pagine: 1826 - 1834
SICI:
0006-3363(200106)64:6<1826:GH(REA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEVELOPMENTALLY RELATED CHANGES; DEVELOPMENT IN-VITRO; NUCLEAR TRANSLOCATION; SOMATOMEDIN-C; FETAL; EMBRYOS; INVITRO; MOUSE; METABOLISM; PROTEIN;
Keywords:
conceptus; developmental biology; early development; growth hormone; IVF/ART;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Kolle, S Univ Munich, Dept Vet Anat 2, Vet Str 13, D-80539 Munich, GermanyUniv Munich Vet Str 13 Munich Germany D-80539 39 Munich, Germany
Citazione:
S. Kolle et al., "Growth hormone (GH)/GH receptor expression and GH-mediated effects during early bovine embryogenesis", BIOL REPROD, 64(6), 2001, pp. 1826-1834

Abstract

Pituitary growth hormone (GH) stimulates postnatal growth and metabolism. The role of CH and its receptor (GHR) during prenatal development, however,is still controversial. As shown by reverse transcription polymerase chainreaction (RT-PCR), bovine in vitro fertilization embryos synthesized the transcript of GHR from Day 2 of embryonic life onwards. Real time RT-PCR revealed that synthesis of GHR mRNA was increased 5.9-fold in 6-day-old embryos compared with 2-day-old embryos. Using in situ hybridization, the mRNA encoding GHR was predominantly localized to the inner cell mass of blastocysts. The GHR protein was first visualized 3 days after fertilization. GH-specific transcripts were first detected in embryos on Day 8 of in vitro culture. As shown by transmission electron microscopy, GH treatment resulted in elimination of glycogen storage in 6- to 8-day-old embryos and an increase in exocytosis of lipid vesicles. These results suggest that a functional GHRable to modulate carbohydrate and lipid metabolism is synthesized during preimplantation development of the bovine embryo and that this GHR may be subject to activation by embryonic GH after Day 8.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:13:40