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Titolo:
Epigenetics of latent Epstein-Barr virus genomes: High resolution methylation analysis of the bidirectional promoter region of latent membrane protein 1 and 2B genes
Autore:
Takacs, M; Salamon, D; Myohanen, S; Li, H; Segesdi, J; Ujvari, D; Uhlig, J; Niller, HH; Wolf, H; Berencsi, G; Minarovits, J;
Indirizzi:
Natl Ctr Epidemiol, Microbiol Res Grp, H-1529 Budapest, Hungary Natl Ctr Epidemiol Budapest Hungary H-1529 Grp, H-1529 Budapest, Hungary Natl Ctr Epidemiol, Div Virol, H-1097 Budapest, Hungary Natl Ctr Epidemiol Budapest Hungary H-1097 rol, H-1097 Budapest, Hungary Semmelweis Univ, Fac Med, Dept Pathol 2, H-1091 Budapest, Hungary Semmelweis Univ Budapest Hungary H-1091 thol 2, H-1091 Budapest, Hungary Univ Kuopio, Dept Biochem & Biotechnol, FIN-70211 Kuopio, Finland Univ Kuopio Kuopio Finland FIN-70211 otechnol, FIN-70211 Kuopio, Finland Hubei Med Univ, Inst Virol, Wuhan 430071, Peoples R China Hubei Med Univ Wuhan Peoples R China 430071 uhan 430071, Peoples R China Budapest Univ Technol & Econ, Dept Agr Chem Technol, H-1111 Budapest, Hungary Budapest Univ Technol & Econ Budapest Hungary H-1111 1 Budapest, Hungary Univ Regensburg, Dept Microbiol & Hyg, D-93053 Regensburg, Germany Univ Regensburg Regensburg Germany D-93053 , D-93053 Regensburg, Germany
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 4, volume: 382, anno: 2001,
pagine: 699 - 705
SICI:
1431-6730(200104)382:4<699:EOLEVG>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHENOTYPE-DEPENDENT CONTROL; NUCLEAR ANTIGEN-2; HISTONE DEACETYLASE; BINDING-PROTEIN; TRANSCRIPTION FACTORS; REGULATORY REGION; DNA METHYLATION; CIS-ELEMENT; J-KAPPA; EXPRESSION;
Keywords:
bisulfite modification; Burkitt's lymphoma; lymphoblastoid cell line; promoter methylation; viral latency;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Minarovits, J Natl Ctr Epidemiol, Microbiol Res Grp, Piheno 1, H-1529 Budapest, Hungary Natl Ctr Epidemiol Piheno 1 Budapest Hungary H-1529 Hungary
Citazione:
M. Takacs et al., "Epigenetics of latent Epstein-Barr virus genomes: High resolution methylation analysis of the bidirectional promoter region of latent membrane protein 1 and 2B genes", BIOL CHEM, 382(4), 2001, pp. 699-705

Abstract

We analysed the methylation patterns of CpG dinucleotides in a bidirectional promoter region (LRS, LMP 1 regulatory sequences) of latent Epstein-Barrvirus (EBV) genomes using automated fluorescent genomic sequencing after bisulfite-induced modification of DNA, Transcripts for two latent membrane proteins, LMP 1 (a transforming protein) and LMP 2B, are initiated in this region in opposite directions. We found that B cell lines and a clone expressing LMP 1 carried EBV genomes with unmethylated or hypomethylated LRS, while highly methylated CpG dinucleotides were present at each position or at discrete sites and within hypermethylated regions in LMP 1 negative cells. Comparison of high resolution methylation maps suggests that CpG methylation-mediated direct interference with binding of nuclear factors LBF 2, 3, 7,AML1/LBF1, L8F5 and LBF6 or methylation of CpGs within an E-box sequence (where activators as well as repressors can bind) is not the major mechanismin silencing of the LMP 1 promoter. Although a role for CPG methylation within binding sites of Sp1 and 3, ATF/CRE and a sis-inducible factor (SIF) cannot be excluded, hypermethylation of LRS or regions within LRS in LMP 1 negative cells suggests a role for an indirect mechanism, via methylcytosinebinding proteins, in silencing of the LMP 1 promoter.

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Documento generato il 04/04/20 alle ore 10:57:24