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Titolo:
C-terminal peptides of interleukin-6 modulate the expression of junB protooncogene and the production of fibrinogen by HepG2 cells
Autore:
Igaz, P; Bosze, S; Toth, S; Falus, A; Hudecz, F;
Indirizzi:
Lorand Eotvos Univ, Hungarian Acad Sci, Res Grp Peptide Chem, H-1518 Budapest, Hungary Lorand Eotvos Univ Budapest Hungary H-1518 hem, H-1518 Budapest, Hungary Semmelweis Univ, Sch Med, Dept Genet Cell & Immunobiol, H-1445 Budapest, Hungary Semmelweis Univ Budapest Hungary H-1445 nobiol, H-1445 Budapest, Hungary
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 4, volume: 382, anno: 2001,
pagine: 669 - 676
SICI:
1431-6730(200104)382:4<669:CPOIMT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT HUMAN INTERLEUKIN-6; HEPATOCYTE-STIMULATING FACTOR; NUCLEAR-MAGNETIC-RESONANCE; TERTIARY STRUCTURAL MODELS; RECEPTOR-BINDING; HUMAN IL-6; MONOCLONAL-ANTIBODIES; SYNTHETIC PEPTIDES; CIRCULAR-DICHROISM; ESCHERICHIA-COLI;
Keywords:
acute phase protein; circular dichroism spectroscopy; HepG2; IL-6 related synthetic peptides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Hudecz, F Lorand Eotvos Univ, Hungarian Acad Sci, Res Grp Peptide Chem, 112,POB 32, H-1518 Budapest, Hungary Lorand Eotvos Univ 112,POB 32 Budapest Hungary H-1518 , Hungary
Citazione:
P. Igaz et al., "C-terminal peptides of interleukin-6 modulate the expression of junB protooncogene and the production of fibrinogen by HepG2 cells", BIOL CHEM, 382(4), 2001, pp. 669-676

Abstract

Interleukin-6 (IL-6) is a 185 amino acid residue helical cytokine with various biological activities (e. g. B cell development, acute phase reaction). We have investigated the role of the 168-185 C-terminal region of IL-6 inthe induction of fibrinogen synthesis and expression of junB mRNA using synthetic peptides corresponding to this region. Circular dichroism spectroscopy data suggest that even truncated peptides have a strong tendency to adopt an ordered conformation. Peptides were tested alone or in combination with recombinant hIL-6 on an IL-6 responsive human hepatoma HepG2 cell line. The expression of the protooncogene junB monitored by competitive RT-PCR represents an early, while the fibrinogen production detected by sandwich ELISA a late, marker of IL-6 initiated events. We found that peptides - depending on their structure - modulate spontaneous as well as IL-6 induced fibrinogen production and/or mRNA expression of junB by exhibiting inhibition (in the presence of IL-6) or stimulation (in the absence of IL-6).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 01:14:19