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Titolo:
Revealing the potential of DNA-based vaccination: Lessons learned from thehepatitis B virus surface antigen
Autore:
Schirmbeck, R; Reimann, J;
Indirizzi:
Univ Ulm, Inst Med Microbiol & Immunol, D-89081 Ulm, Germany Univ Ulm Ulm Germany D-89081 d Microbiol & Immunol, D-89081 Ulm, Germany
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 4, volume: 382, anno: 2001,
pagine: 543 - 552
SICI:
1431-6730(200104)382:4<543:RTPODV>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOTOXIC T-LYMPHOCYTE; RESTRICTED EPITOPE PRESENTATION; HUMORAL IMMUNE-RESPONSES; PLASMID DNA; IN-VIVO; GENETIC IMMUNIZATION; MEDIATED IMMUNIZATION; ANTIBODY-RESPONSES; MESSENGER-RNA; PROTECTIVE EFFICACY;
Keywords:
antibody responses; CD8(+) cytotoxic T lymphocytes; DNA-based vaccines; expression vectors; hepatitis B virus;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Schirmbeck, R Univ Ulm, Inst Med Microbiol & Immunol, D-89081 Ulm, GermanyUniv Ulm Ulm Germany D-89081 Immunol, D-89081 Ulm, Germany
Citazione:
R. Schirmbeck e J. Reimann, "Revealing the potential of DNA-based vaccination: Lessons learned from thehepatitis B virus surface antigen", BIOL CHEM, 382(4), 2001, pp. 543-552

Abstract

DNA-based vaccination is a novel technique to efficiently stimulate humoral (antibody) and cellular (T cell) immune responses to protein antigens. InDNA-based vaccination, immunogenic proteins are expressed in in vivo transfected cells of the vaccine recipients in their native conformation with correct posttranslational modifications from antigen-encoding expression plasmid DNA, This ensures the integrity of antibody-defined epitopes and supports the generation of protective (neutralizing) antibody titers. Plasmid DNAvaccination is furthermore an exceptionally potent strategy to stimulate CD8(+) cytotoxic T lymphocyte (CTL) responses because antigenic peptides areefficiently generated by endogenous processing of intracellular protein antigens. These key features make DNA-based immunization an attractive strategy for prophylactic and therapeutic vaccination against extra- and intracellular pathogens. In this brief review, we summarize the current state of expression vector design, DNA delivery strategies, priming immune responses to intracellular or secreted antigens by DNA vaccines and unique advantages of DNA- versus recombinant protein-based vaccines using the hepatitis B surface antigen (HBsAS) as a model antigen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 16:06:40