Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Water-soluble lipopolymer for gene delivery
Autore:
Han, SO; Mahato, RI; Kim, SW;
Indirizzi:
Univ Utah, Ctr Controlled Chem Delivery, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 Chem, Salt Lake City, UT 84112 USA
Titolo Testata:
BIOCONJUGATE CHEMISTRY
fascicolo: 3, volume: 12, anno: 2001,
pagine: 337 - 345
SICI:
1043-1802(200105/06)12:3<337:WLFGD>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMID DNA; LIPOSOME COMPLEXES; CATIONIC POLYMERS; MAMMALIAN-CELLS; IN-VIVO; EFFICIENT; POLYETHYLENIMINE; TRANSFECTION; EXPRESSION; LIPIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, SW Univ Utah, Ctr Controlled Chem Delivery, Dept Pharmaceut & Pharmaceut Chem, BPRB,Room 205, Salt Lake City, UT 84112 USA Univ Utah BPRB,Room 205 Salt Lake City UT USA 84112 , UT 84112 USA
Citazione:
S.O. Han et al., "Water-soluble lipopolymer for gene delivery", BIOCONJ CHE, 12(3), 2001, pp. 337-345

Abstract

The use of biocompatible polymeric gene carriers may overcome the current problems associated with viral vectors in safety, immunogenicity, and mutagenesis. Nontoxic water-soluble lipopolymer (WSLP), poly{(ethylenimine)-co-[N-(2-aminoethyl) ethyleneimin]-co-N-(N-cholesteryloxycarbonyl-(2-aminoethyl)ethylenimine)} was synthesized using branched poly(ethylenimine) (PEI, mw 1800) and cholesteryl chloroformate. Following synthesis and purification, the structure and molecular weight of WSLP were confirmed by H-1 NMR and MADI-TOF mass spectrometry, respectively. The percentage of cholesterol conjugated to PEI was about 47%, and the average molecular weight of WSLP was approximately 2000 Da. WSLP/pDNA complexes were prepared at different N/P (nitrogen atoms of WSLP/phosphate of plasmid DNA) ratios and characterized in terms of particle size, zeta potential, osmolarity, surface morphology, andcytotoxicity. WSLP condensed plasmid DNA when N/P ratio reached 2.5/1 and no free DNA was detected at N/P ratio of 5/1 and above, as determined by agarose gel electrophoresis. The mean particle size was in the range of 25.9 to 148.5 nm and was dependent on N/P ratios. Atomic force microscopy (AFM) showed complete condensation of plasmid DNA with spherical particles of similar to 50 nm in diameter. WSLP/pDNA complexes or WSLP itself were nontoxicto CT-26 colon adenocarcinoma and 293 T human embryonic kidney transformedcells when formulated at the N/P ratio of 10/1 and below as determined by MTT assay. In contrast, PEI25000/pDNA complexes were toxic to these cells. Erythrocytes aggregated when incubated with PEI25000/pCMV-Luc complexes at high DNA concentrations, but there was little aggregation with WSLP/pCMV-Luc complexes. WSLP/pCMV-Luc complexes demonstrated higher transfection efficiency in both CT-26 and 293 T cells compared to PEI25000- or PEI1800-based formulations. WSLP/pCMV-Luc complexes are nontoxic and showed enhanced in vitro transfection. Thus, WSLP will be a suitable carrier for in vivo gene delivery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 09:20:10