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Titolo:
Critical sites for the interaction between IL-2R gamma and JAK3 and the following signaling
Autore:
Tang, W; Hue, HR; Zhu, JF; Ji, HB; Zou, WG; Xu, LF; Sun, LY; Zheng, ZC; Theze, J; Liu, XY;
Indirizzi:
Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China Chinese Acad Sci Shanghai Peoples R China 200031 200031, Peoples R China Inst Pasteur, Paris, France Inst Pasteur Paris FranceInst Pasteur, Paris, France
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 283, anno: 2001,
pagine: 598 - 605
SICI:
0006-291X(20010511)283:3<598:CSFTIB>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-GAMMA-CHAIN; MEMBRANE-PROXIMAL REGION; IL-2 RECEPTOR; BETA-CHAIN; FUNCTIONAL COMPONENT; KINASE ACTIVATION; PROTEIN-KINASE; C-MYC; TRANSDUCTION; INDUCTION;
Keywords:
IL-2 R gamma; JAK3; yeast two-hybrid system; signal transduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, XY Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 YueYang Rd, Shanghai 200031, Peoples R China Chinese Acad Sci 320 YueYang Rd Shanghai Peoples R China 200031 a
Citazione:
W. Tang et al., "Critical sites for the interaction between IL-2R gamma and JAK3 and the following signaling", BIOC BIOP R, 283(3), 2001, pp. 598-605

Abstract

JAK3 is the only known protein tyrosine kinase associating with IL-2R gamma. This interaction is supposed to be very important to IL-2 signaling. In order to identify the critical residues for these two molecular interactions and the following signal events, various mutants of gammac and JAK3 were constructed on the basis of computer analysis. The direct interaction was determined via the yeast two-hybrid system, while the signaling was analyzedwith reporter genes under the control of the c-fos, c-myc, or tnf-beta promoters, respectively. Results showed that there are two key sites on gammacinvolved in this interaction and the following signal transduction: the critical one is E327 via electrostatic interaction, the other is L293 via hydrophobic interaction. As to JAK3, the data indicated that Y100 is importantfor the interaction with gammac. These results also document that the requirement for interaction between gammac and JAK3 is different to activate different signaling pathways mediated by gammac, such as c-fos, c-myc, and JAK-STAT. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:24:09