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Titolo:
Identification and characterization of a sodium/calcium exchanger, NCX-1, in osteoclasts and its role in bone resorption
Autore:
Moonga, BS; Davidson, R; Sun, L; Adebanjo, OA; Moser, J; Abedin, M; Zaidi, N; Huang, CLH; Zaidi, M;
Indirizzi:
Mt Sinai Sch Med, Mt Sinai Bone Program, Dept Med, New York, NY 10029 USA Mt Sinai Sch Med New York NY USA 10029 , Dept Med, New York, NY 10029 USA Mt Sinai Sch Med, Mt Sinai Bone Program, Dept Geriatr, New York, NY 10029 USA Mt Sinai Sch Med New York NY USA 10029 pt Geriatr, New York, NY 10029 USA Bronx Vet Affairs Geriatr Res Educ & Clin Ctr, New York, NY USA Bronx Vet Affairs Geriatr Res Educ & Clin Ctr New York NY USA rk, NY USA NYU, David Kriser Sch Dent, New York, NY USA NYU New York NY USANYU, David Kriser Sch Dent, New York, NY USA Univ Pittsburgh, Dept Surg, Pittsburgh, PA USA Univ Pittsburgh PittsburghPA USA tsburgh, Dept Surg, Pittsburgh, PA USA Univ Cambridge, Physiol Lab, Cambridge CB2 3EG, England Univ Cambridge Cambridge England CB2 3EG Lab, Cambridge CB2 3EG, England Vet Affairs Med Ctr, Philadelphia, PA USA Vet Affairs Med Ctr Philadelphia PA USA rs Med Ctr, Philadelphia, PA USA
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 4, volume: 283, anno: 2001,
pagine: 770 - 775
SICI:
0006-291X(20010518)283:4<770:IACOAS>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR ACTIVATION; INTRACELLULAR PH; RAT OSTEOCLASTS; CYTOPLASMIC PH; NEONATAL RAT; CALCIUM; CALCITONIN; TRANSPORT; MECHANISM; PUMPS;
Keywords:
Na+/Ca2+ exchange; osteoclasts; osteoporosis; bone resorption;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Zaidi, M Mt Sinai Sch Med, Mt Sinai Bone Program, Dept Med, POB 1055,1 Gustav Levy Pl, New York, NY 10029 USA Mt Sinai Sch Med POB 1055,1 Gustav LevyPl New York NY USA 10029
Citazione:
B.S. Moonga et al., "Identification and characterization of a sodium/calcium exchanger, NCX-1, in osteoclasts and its role in bone resorption", BIOC BIOP R, 283(4), 2001, pp. 770-775

Abstract

We provide the first demonstration for a Na+/Ca2+ exchanger, NCX-1, in theosteoclast. We speculate that by using Na+ exchange, NCX-1 couples H+ extrusion with Ca2+ fluxes during bone resorption. Microspectrofluorimetry of fura-a-loaded osteoclasts revealed a rapid and sustained, but reversible, cytosolic Ca2+ elevation upon Na+ withdrawal. This elevation was abolished bythe cytosolic introduction (by gentle permeabilization) of a highly specific Na+/Ca2+ exchange inhibitor peptide, XIP, but not its inactive analogue,sXIP. Confocal microscopy revealed intense plasma membrane immunofluorescence with an isoform-specific monoclonal anti-NCX-1 antibody applied to gently permeabilized osteoclasts. Electrophysiological studies using excised outside-in membrane patches showed a low-conductance, Na+-selective, dichlorobenzamil-sensitive, amiloride-insensitive channel that we tentatively assigned as being an NCX. Finally, to examine for physiological relevance, an osteoclast resorption (pit) assay was performed. There was a dramatic reduction of bone resorption following NCX-1 inhibition by dichlorobenzamil and XIP (but not with S-XIP). Together, the results suggest that a functional NCX, likely NCX-1, is involved in the regulation of osteoclast cytosolic Ca2+ and bone resorption. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:12:19