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Titolo:
Formalin-fixed and heat-retrieved tissue antigens: A comparison of their immunoreactivity in experimental antibody diluents
Autore:
Boenisch, T;
Indirizzi:
DAKO Corp, Carpinteria, CA 93013 USA DAKO Corp Carpinteria CA USA 93013DAKO Corp, Carpinteria, CA 93013 USA
Titolo Testata:
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
fascicolo: 2, volume: 9, anno: 2001,
pagine: 176 - 179
SICI:
1062-3345(200106)9:2<176:FAHTAA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITOPE RETRIEVAL; IMMUNOHISTOCHEMISTRY; STANDARDIZATION; PERFORMANCE; SECTIONS; BUFFER;
Keywords:
antigen retrieval; immunohistochemistry; molecular structure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Boenisch, T DAKO Corp, 6392 Via Real, Carpinteria, CA 93013 USA DAKO Corp 6392 Via Real Carpinteria CA USA 93013 CA 93013 USA
Citazione:
T. Boenisch, "Formalin-fixed and heat-retrieved tissue antigens: A comparison of their immunoreactivity in experimental antibody diluents", APPL IMMUNO, 9(2), 2001, pp. 176-179

Abstract

Heat-induced antigen retrieval procedures have become an effective and therefore important part of immunohistochemistry. Although used with increasing frequency, it is not known by what mechanism or to what extent these procedures restore the molecular integrity of the antigen. In an attempt to explore these questions, the immune reactions of 14 tissue antigens with theircorresponding monoclonal antibodies were compared both in the absence and after the application of two widely used heat-induced antigen retrieval procedures. The tissue antigens were selected either because of the increased or lack of response to heat retrieval. The nature of their immune reactionswas appraised through the variable effects that some diluent solutes were previously shown to have on monoclonal antibodies at different pH. It was found that on heat retrieval, four antigens reacting with monoclonal antibodies of subclass IgG2a or IgG1 reversed their preference for an alkaline pH to that of an acid pH. Eight of nine antigens reacting with monoclonal antibodies of subclass IgG1 and one antigen reacting with an antibody of class IgM remained most reactive at pH 6.0. The retrieved epitope of a B-cell antigen reacting with an IgG3 subclass antibody retained its preference for the alkaline pH. These patterns of response by the antigens to heat-induced retrieval are compared with the previously reported modifications in the immune reactions of monoclonal antibodies when sodium chloride and sodium phosphates were included in the antibody diluent buffer. Use of antibody diluents containing these solutes in combination with heat-induced antigen retrieval resulted in the loss of most of the sensitivity gained through this technique. The possible common mechanisms underlying the causes for these results are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:38:24