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Titolo:
MMP-3 mRNA and MMP-3 and MMP-1 proteins in bladder cancer: A comparison with clinicopathologic features and survival
Autore:
Nakopoulou, L; Gakiopoulou, H; Zervas, A; Giannopoulou, I; Constantinides, C; Lazaris, AC; Liapis, H; Kyriakou, G; Dimopoulos, C;
Indirizzi:
Univ Athens, Dept Pathol, Sch Med, GR-11527 Athens, Greece Univ Athens Athens Greece GR-11527 hol, Sch Med, GR-11527 Athens, Greece Univ Athens, Dept Urol, Sch Med, GR-11527 Athens, Greece Univ Athens Athens Greece GR-11527 rol, Sch Med, GR-11527 Athens, Greece Washington Univ, Dept Pathol, St Louis, MO USA Washington Univ St Louis MO USA gton Univ, Dept Pathol, St Louis, MO USA
Titolo Testata:
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
fascicolo: 2, volume: 9, anno: 2001,
pagine: 130 - 137
SICI:
1062-3345(200106)9:2<130:MMAMAM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARCINOMA CELL-LINES; MATRIX METALLOPROTEINASE-1; POOR-PROGNOSIS; MESSENGER-RNA; TUMOR-CELLS; TISSUE; EXPRESSION; COLLAGENASE; MECHANISMS; BREAST;
Keywords:
MMP-1 protein; MMP-3 protein; MMP-3 mRNA; bladder cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Nakopoulou, L Univ Athens, Dept Pathol, Sch Med, 75 M Assias Str, GR-11527Athens, Greece Univ Athens 75 M Assias Str Athens Greece GR-11527 , Greece
Citazione:
L. Nakopoulou et al., "MMP-3 mRNA and MMP-3 and MMP-1 proteins in bladder cancer: A comparison with clinicopathologic features and survival", APPL IMMUNO, 9(2), 2001, pp. 130-137

Abstract

Matrix metalloproteinases (MMPs) are proteolytic enzymes important at several points during multistep neoplastic progression. Although MMP-1 and MMP-3 have been implicated in the progression of various human cancers, their expression in bladder cancer has not been addressed. Immunohistochemistry (Strept-ABC-HRP method) and in situ hybridization were performed to detect MMP-1 protein, MMP-3 protein, and MMP-3 mRNA, respectively, in 59 transitional cell bladder carcinomas. To assess the role of these MMPs in bladder cancer, their expression was evaluated in relation to known clinicopathologic parameters and patients' disease-free and overall survival. Immunoreactivityfor MMP-1 and MMP-3 proteins was observed in the cytoplasm of cancer cellsin 30.5% and 24% of samples, respectively. Transcripts for MMP-3 mRNA werelocalized in stromal cells in 71.2% of cases and in cancer cells in 49% ofcases. MMP-1 immunoreactivity demonstrated a statistically significant association with deeply invasive and grade III tumors versus superficial and lower grade tumors. MMP-3 protein immunoreactivity and MMP-3 mRNA immunolocalization did not associate with the parameters studied. However, MMP-3 mRNAlocalization in stromal cells demonstrated a borderline association with poor patients' disease-free and overall survival. In conclusion, the authors' results demonstrate a differential expression between MMP-1 and MMP-3 in bladder cancer; MMP-1 appears to participate in invasiveness and possibly in loss of differentiation in urothelial carcinomas in contrast to MMP-3.

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Documento generato il 29/03/20 alle ore 15:13:32