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Titolo:
Development of aflatoxin B-1-lysine adduct monoclonal antibody for human exposure studies
Autore:
Wang, JS; Abubaker, S; He, X; Sun, GJ; Strickland, PT; Groopman, JD;
Indirizzi:
Texas Tech Univ, Inst Environm & Human Hlth, Lubbock, TX 79409 USA Texas Tech Univ Lubbock TX USA 79409 & Human Hlth, Lubbock, TX 79409 USA Texas Tech Univ, Dept Environm Toxicol, Lubbock, TX 79409 USA Texas Tech Univ Lubbock TX USA 79409 ironm Toxicol, Lubbock, TX 79409 USA Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Environm Hlth Sci, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 th Sci, Baltimore, MD 21205 USA
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 6, volume: 67, anno: 2001,
pagine: 2712 - 2717
SICI:
0099-2240(200106)67:6<2712:DOABAM>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPUBLIC-OF-CHINA; SERUM-ALBUMIN ADDUCT; HEPATOCELLULAR-CARCINOMA; MOLECULAR EPIDEMIOLOGY; CHEMICAL CARCINOGENS; BIOMARKERS; QIDONG; QUANTITATION; RESIDENTS; AFFINITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, JS Texas Tech Univ, Inst Environm & Human Hlth, Box 41163, Lubbock, TX 79409 USA Texas Tech Univ Box 41163 Lubbock TX USA 79409 bock, TX 79409 USA
Citazione:
J.S. Wang et al., "Development of aflatoxin B-1-lysine adduct monoclonal antibody for human exposure studies", APPL ENVIR, 67(6), 2001, pp. 2712-2717

Abstract

Mouse monoclonal antibodies were developed against a synthetic aflatoxin B, (AFB)-lysine-cationized bovine serum albumin conjugate, The isotype of one of these antibodies, IIA4B3, has been classified as immunoglobulin Gl(h),The affinity and specificity of IIA4B3 were further characterized by a competitive radioimmunoassay. The affinities of IIA4B3 for AFB and its associated adducts and metabolites are ranked as follows: AFB-lysine > 8,9-dihydro-8-(2,6-diamino-4-oxo-3,4-dihydropyrimid-5-yl formamido)-9-hydnEy-AFB > AFB= 8,9-dihydro-8-(N-7-guanyl)-9-hydroxy-AFB > aflatoxin M-1 > aflatoxin Q(1). IIA4B3 had about a 10-fold higher affinity for binding to AFB-lysine adduct than to AFB when H-3-AFB-lysine was used as the tracer. The concentration for 50% inhibition for AFB-lysine was 0.610 pmol; that for AFB was 6.85 pmol, IIA4B3 had affinities at least sevenfold and twofold higher than those of 2B11, a previously developed antibody against parent AFB, for the major aflatoxin-DNA adducts 8,9-dihydro-8-(N-7-guanyl)-9-hydroxy-AFB and 8,9-dihydro-8-(2,6-diamino-4-oxo-3,4-dihydropyrimid-5-yl formamido)-9-hydroxy-AFB, respectively. An analytical method based on a competitive radioimmunoassay with IIA4B3 and H-3-AFB-lysine was validated with a limit of detection of10 fmol of AFB-lysine adduct, The method has been applied to the measurement of AFB-albumin adduct levels in human serum samples collected from the residents of areas at high risk for liver cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:45:10