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Titolo:
Fluorogenic assay for penicillin G acylase activity
Autore:
Ninkovic, M; Riester, D; Wirsching, F; Dietrich, R; Schwienhorst, A;
Indirizzi:
Inst Mikrobiol & Genet, Abt Mol Genet & Praeparat Mol Biol, D-37077 Gottingen, Germany Inst Mikrobiol & Genet Gottingen Germany D-37077 7077 Gottingen, Germany
Titolo Testata:
ANALYTICAL BIOCHEMISTRY
fascicolo: 2, volume: 292, anno: 2001,
pagine: 228 - 233
SICI:
0003-2697(20010515)292:2<228:FAFPGA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATRIX METALLOPROTEINASES; AMIDASE ACTIVITY; SUBSTRATE; CHYMOTRYPSIN; INHIBITION; BACTERIA; PLASMA; ACID;
Keywords:
fluorogenic substrates; high-throughput screening assay; penicillin G acylase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Schwienhorst, A Inst Mikrobiol & Genet, Abt Mol Genet & Praeparat Mol Biol, Grisebachstr 8, D-37077 Gottingen, Germany Inst Mikrobiol & Genet Grisebachstr 8 Gottingen Germany D-37077
Citazione:
M. Ninkovic et al., "Fluorogenic assay for penicillin G acylase activity", ANALYT BIOC, 292(2), 2001, pp. 228-233

Abstract

A simple, highly sensitive, and rapid assay for high-throughput screening of penicillin G acylase-producing bacteria is presented. The method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin G acylase. The present method is suitable for screening pure enzymes as well as various penicillin G acylases like those from Escherichia coli, Proteus rettgeri, and Kluyvera citrophila in cell extracts. In addition, the new substrate was used for rapid assay of amidase activity in nondenaturing polyacrylamide gels. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 16:16:11