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Titolo:
A direct sensitized fluorimetric determination of 5,10,15,20-tetra(m-hydroxyphenyl)chlorin [m-THPC (Foscan)(R)] in human plasma using a cyclodextrin inclusion complex
Autore:
Desroches, MC; Kasselouri, A; Bourdon, O; Chaminade, P; Blais, J; Prognon, P;
Indirizzi:
Univ Paris 11, Fac Pharm, Chim Analyt Lab, F-92296 Chatenay Malabry, France Univ Paris 11 Chatenay Malabry France F-92296 6 Chatenay Malabry, France Univ Paris 06, CNRS, ESA 7033, Lab Physicochim Biomol & Cellulaire, F-75252 Paris, France Univ Paris 06 Paris France F-75252 l & Cellulaire, F-75252 Paris, France
Titolo Testata:
ANALYST
fascicolo: 6, volume: 126, anno: 2001,
pagine: 923 - 927
SICI:
0003-2654(2001)126:6<923:ADSFDO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOTODYNAMIC THERAPY; TEMOPORFIN; FLUORESCENCE; PHARMACOKINETICS; PHOTOSENSITIZER; CANCER; TUMOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Prognon, P Univ Paris 11, Fac Pharm, Chim Analyt Lab, 5 Rue JB Clement, F-92296 Chatenay Malabry, France Univ Paris 11 5 Rue JB Clement Chatenay Malabry France F-92296
Citazione:
M.C. Desroches et al., "A direct sensitized fluorimetric determination of 5,10,15,20-tetra(m-hydroxyphenyl)chlorin [m-THPC (Foscan)(R)] in human plasma using a cyclodextrin inclusion complex", ANALYST, 126(6), 2001, pp. 923-927

Abstract

The 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (m-THPC) (Foscan (R)) is a photosensitizer used in the photodynamic therapy (PDT) of cancers which is currently under clinical trial. The formation of a m-THPC inclusion complex with dimethyl-beta -cyclodextrin (Me-beta -CD) in solution was demonstrated on the basis of circular dichroism experiments. A 1:2 complex stoichiometrywas found and an inclusion constant beta (2) = 2.8(+/-0.4) x 10(10) M-2 was determined. The formation of such a complex was shown to enhance the m-THPC fluorescence intensity. It could be exploited to improve the sensitivityof the direct m-THPC detection in human plasma. Optimization of the operating conditions shows that the best results were obtained by the addition of100 muL of a concentrated Me-beta -CD solution (3.2 x 10(-2) M) to 1 mL plasma samples. Compared to the standard conditions, a 90% increase in sensitivity was obtained. The proposed analytical method which showed a linear response function [0-300 ng mL(-1) (440 pM)] and a low limit of detection [1.5 ng mL(-1) (2 pM) (S/N = 3)] appears, especially due to the absence of metabolism, a simple and specific method suitable for pharmacokinetics studiesin patients.

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Documento generato il 02/04/20 alle ore 12:44:10