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Titolo:
Human developing motor neurons as a tool to study ALS
Autore:
Silani, V; Braga, M; Botturi, A; Cardin, V; Bez, A; Pizzuti, A; Scarlato, G;
Indirizzi:
Univ Milan, Osped Maggiore, IRCCS,Sch Med, Inst Neurol, I-20122 Milan, Italy Univ Milan Milan Italy I-20122 ch Med, Inst Neurol, I-20122 Milan, Italy
Titolo Testata:
AMYOTROPHIC LATERAL SCLEROSIS AND OTHER MOTOR NEURON DISORDERS
, volume: 2, anno: 2001, supplemento:, 1
pagine: S69 - S76
SICI:
1466-0822(200103)2:<S69:HDMNAA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SPINAL-CORD; DISSOCIATED MONOLAYER-CULTURES; CENTRAL-NERVOUS-SYSTEM; NEURAL STEM-CELL; HUMAN CNS; IN-VITRO; RAT; ESTABLISHMENT; EXPRESSION; DISEASE;
Keywords:
motor neuron; human; tissue culture; ALS; microarray;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Silani, V Univ Milan, Osped Maggiore, IRCCS,Sch Med, Inst Neurol, Via F Sforza 35, I-20122 Milan, Italy Univ Milan Via F Sforza 35 Milan Italy I-20122 122 Milan, Italy
Citazione:
V. Silani et al., "Human developing motor neurons as a tool to study ALS", AMYOTROPH L, 2, 2001, pp. S69-S76

Abstract

Defining the basis of the selective cell vulnerability of human motor neurons (hMNs) represents a crucial step in revealing the pathogenesis of amyotrophic lateral sclerosis (ALS), Tissue culture models offer an ideal systemfor identification of the hMN-specific features at the single cell level. Purified hMNs and astrocytes can today be isolated from the anterior horn of the human embryonic spinal cord. Cultures can be studied at the single cell level using cDNA/mRNA amplification techniques. The effects of moleculesaffecting hMN survival, neurite extension, and metabolism can be tested invitro and the expression of selective genes assayed using DNA microarray technology. Crucial information of immediate clinical application for the treatment of patients affected by ALS can be derived after testing the efficacy of candidate pharmaceutical molecules using in vitro cell models. Adult nervous tissue or progenitor cells derived from different regions of the nervous system may be used as an alternative source of human neuronal cells. HMNs in culture, combined with the application of adequate technology, can contribute greatly to identifying the primitive critical events responsiblefor the cell degeneration observed in ALS, bypassing the intrinsic limitations of the non-human models of the disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 09:49:25