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Titolo:
DNA amplification for the diagnosis of cat-scratch disease in small-quantity clinical specimens
Autore:
Avidor, B; Varon, M; Marmor, S; Lifschitz-Mercer, B; Kletter, Y; Ephros, M; Giladi, M;
Indirizzi:
Tel Aviv Sourasky Med Ctr, Dept Pathol, IL-64239 Tel Aviv, Israel Tel AvivSourasky Med Ctr Tel Aviv Israel IL-64239 4239 Tel Aviv, Israel Tel Aviv Sourasky Med Ctr, Bernard Pridan Lab Mol Biol Infect Dis, IL-64239 Tel Aviv, Israel Tel Aviv Sourasky Med Ctr Tel Aviv Israel IL-64239 4239 Tel Aviv, Israel Carmel Med Ctr, Dept Pediat, Haifa, Israel Carmel Med Ctr Haifa IsraelCarmel Med Ctr, Dept Pediat, Haifa, Israel
Titolo Testata:
AJCP. American journal of clinical pathology
fascicolo: 6, volume: 115, anno: 2001,
pagine: 900 - 909
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; PARAFFIN-EMBEDDED TISSUES; BARTONELLA-HENSELAE DNA; ROCHALIMAEA-HENSELAE; SEROLOGICAL RESPONSE; MOLECULAR DIAGNOSIS; PCR; ANTIGENS; SKIN; IDENTIFICATION;
Keywords:
cat-scratch disease; polymerase chain reaction; Bartonella henselae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Avidor, B Tel Aviv Sourasky Med Ctr, Ichilov Hosp, Lab Mol Biol Infect Dis, 6 Weizman St, IL-64239 Tel Aviv, Israel Tel Aviv Sourasky Med Ctr 6 Weizman St Tel Aviv Israel IL-64239
Citazione:
B. Avidor et al., "DNA amplification for the diagnosis of cat-scratch disease in small-quantity clinical specimens", AM J CLIN P, 115(6), 2001, pp. 900-909

Abstract

Diagnosis of cat-scratch disease (CSD) by polymerase chain reaction (PCR) of lymph node fine-needle aspiration (FNA) and primary lesion specimens canhe difficult owing to the minute amount of available material. A PCR assayspecifically suited to test these specimens was developed. First, small-quantity (10 muL) samples were prepared from 17 CSD-positive and 16 CSD-negative specimens, and DNA extraction and amplification from these samples werecompared using 3 methods. Sensitivity and specificity of PCR were 100% using material collected on glass microscope slides and by using Qiagen (Hilden, Germany) columns for DNA extraction. Then, this method was used to test II archival glass microscope slides of FNA (7 malignant neoplasms, 4 undiagnosed lymphadenitis) and 2 primary lesion specimens. Two of the 4 lymphadenitis samples and the 2 primary lesion specimens were PCR positive. The technique presented could facilitate CSD diagnosis from a wider range of clinical samples.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 10:16:59