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Titolo:
Age-related changes in cytokine production by leukocytes in rhesus monkeys
Autore:
Mascarucci, P; Taub, D; Saccani, S; Paloma, MA; Dawson, H; Roth, GS; Ingram, DK; Lane, MA;
Indirizzi:
NIA, Gerontol Res Ctr, Lab Neurosci, NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224 tr, Lab Neurosci, NIH, Baltimore, MD 21224 USA
Titolo Testata:
AGING-CLINICAL AND EXPERIMENTAL RESEARCH
fascicolo: 2, volume: 13, anno: 2001,
pagine: 85 - 94
SICI:
0394-9532(200104)13:2<85:ACICPB>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD MONONUCLEAR-CELLS; PERIPHERAL-BLOOD; IMMUNE-SYSTEM; DIETARY RESTRICTION; NONHUMAN-PRIMATES; INTERFERON-GAMMA; WHOLE-BLOOD; IFN-GAMMA; EXPRESSION; IMMUNOSENESCENCE;
Keywords:
IL-1; IL-6; IL-10; IFN gamma; TNF alpha; rhesus monkey; PBMCs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Lane, MA NIA, Gerontol Res Ctr, Lab Neurosci, NIH, 5600 Nathan Shock Dr, Baltimore,MD 21224 USA NIA 5600 Nathan Shock Dr Baltimore MD USA 21224 more,MD 21224 USA
Citazione:
P. Mascarucci et al., "Age-related changes in cytokine production by leukocytes in rhesus monkeys", AGING-CLIN, 13(2), 2001, pp. 85-94

Abstract

Using a variety of experimental rodent and human models, age-related alterations in cytokine production by immune cells have been described extensively. While the precise mechanism(s) responsible for such age-related changesin cytokine responses remain unclear, it seems likely that these changes may have a significant effect on immune cell function. In an attempt to clarify such changes in aging primates, we examined cytokine production by white cells derived from a controlled colony of rhesus monkeys (Macaca mulatta). Non-fractionated whole blood and peripheral blood mononuclear cells (PBMCs) were obtained from male monkeys of different ages (6-28 years), and weresubsequently evaluated for their ability to express mRNA and protein for the cytokines, IL-10, IL-6, IFN gamma, IL-1 beta, and TNF alpha, following in vitro stimulation with polyclonal mitogens. Our results suggest that white blood cells derived from aged rhesus monkeys exhibit a significant increase in their ability to produce the Th2-associated cytokine, IL-10, upon stimulation with lipopolysaccharide (LPS when compared to white cells derived from younger counterparts Similarly, a significant age-related decrease in the expression of the Thlassociated cytokine, IFN gamma, was also observed using phytohemagglutinin (PHA)-stimulated PBMCs. No significant age-relateddifferences in the production of IL-1 beta or TNF alpha were observed in response to any stimulation, but there was limited evidence of an age-related increase in IL-6 production. Overall, our results suggest that a possiblesystemic change from a Th0/Th1 to a Th2-like cytokine profile occurs in circulating leukocytes derived from aging primates. We believe that such age-related alterations in cytokine production may play a role in the reduced immune responses observed in elderly human populations. (C) 2001, Editrice Kurtis.

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Documento generato il 27/01/20 alle ore 15:16:39