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Titolo:
Studies on inhibition of human platelet function by sodium nitroprusside. kinetic evaluation of the effect on aggregation and cyclic nucleotide content
Autore:
Anfossi, G; Russo, I; Massucco, P; Mattiello, L; Balbo, A; Cavalot, F; Trovati, M;
Indirizzi:
Univ Turin, Azienda Osped S Luigi, Dept Clin & Biol Sci, Diabet Unit, I-10043 Orbassano, TO, Italy Univ Turin Orbassano TO Italy I-10043 Unit, I-10043 Orbassano, TO, Italy
Titolo Testata:
THROMBOSIS RESEARCH
fascicolo: 4, volume: 102, anno: 2001,
pagine: 319 - 330
SICI:
0049-3848(20010515)102:4<319:SOIOHP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE RELAXATION; STABLE ANGINA-PECTORIS; NITRIC-OXIDE; SYNERGISTIC INHIBITION; GUANYLATE-CYCLASE; ORGANIC NITRATES; RELAXING FACTOR; NITROGLYCERIN; GMP; MONOPHOSPHATE;
Keywords:
cyclic AMP; cyclic GMP; nitric oxide; sodium nitroprusside; platelet aggregation; zaprinast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Anfossi, G Univ Turin, Azienda Osped S Luigi, Dept Clin & Biol Sci, DiabetUnit, I-10043 Orbassano, TO, Italy Univ Turin Orbassano TO Italy I-10043 043 Orbassano, TO, Italy
Citazione:
G. Anfossi et al., "Studies on inhibition of human platelet function by sodium nitroprusside. kinetic evaluation of the effect on aggregation and cyclic nucleotide content", THROMB RES, 102(4), 2001, pp. 319-330

Abstract

In this study, we explored the ability of sodium nitroprusside to inhibit the aggregation of human platelets in platelet-rich plasma (PRP) and whole blood and its effects on intracellular levels of guanosine 3',5'-cyclic monophosphate (cGMP) and adenosine 3',5'-cyclic monophosphate (cAMP). The experiments investigated dose-dependent effects of nitroprusside starting from concentrations in the range of circulating levels achievable in vivo duringdrug administration in humans. Furthermore, we investigated the timecourseof both antiaggregating action and the influence on cyclic nucleotide synthesis. Results showed that sodium nitroprusside inhibited the aggregation induced by adenosine 5-diphosphate (ADP) and collagen starting from concentration as low as 2 mu mol/l. The IC50 value for ADP-induced aggregation in PRP was 18.7 +/- 2.4 mu mol/l. The inhibition of platelet aggregation showeda time-dependent behaviour and was not reversible within 90 min. The accumulation of intraplatelet cGMP in the presence of sodium nitroprusside exhibited a comparable time-course characterized by an early increase, a steady state and a late further increase. The time-course of cAMP synthesis was very similar to that of cGMP. Our data evidenced a long-lasting inhibition ofplatelet responses by sodium nitroprusside and excluded a desensitization of platelet guanylyl cyclase after 3-h exposure to nitric oxide (NO). Furthermore, they indicated a role of cAMP accumulation in the antiaggregating effects of nitroso donor: the simultaneous increase of intracellular contentof cAMP and cGMP can synergize in the reduction of the platelet responses. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 13:43:36