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Titolo:
7E3 F(ab ')(2), an effective antagonist of rat alpha IIb beta 3 and alpha v beta 3, blocks in vivo thrombus formation and in vitro angiogenesis
Autore:
Sassoli, PM; Emmell, EL; Tam, SH; Trikha, M; Zhou, Z; Jordan, RE; Nakada, MT;
Indirizzi:
Centocor Inc, Biol Res, Malvern, PA 19355 USA Centocor Inc Malvern PA USA19355 or Inc, Biol Res, Malvern, PA 19355 USA
Titolo Testata:
THROMBOSIS AND HAEMOSTASIS
fascicolo: 5, volume: 85, anno: 2001,
pagine: 896 - 902
SICI:
0340-6245(200105)85:5<896:7F'AEA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOPROTEIN-IIB/IIIA RECEPTOR; MONOCLONAL-ANTIBODY; PLATELET-AGGREGATION; IIB-IIIA; FAB; INHIBITION; FRAGMENTS; INTEGRINS; COMPLEX; NUMBER;
Keywords:
platelet aggregation; alpha v beta 3; GPIIb/IIIa; angiogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Nakada, MT Centocor Inc, Biol Res, 200 Great Valley Pkwy, Malvern, PA 19355 USA Centocor Inc 200 Great Valley Pkwy Malvern PA USA 19355 355 USA
Citazione:
P.M. Sassoli et al., "7E3 F(ab ')(2), an effective antagonist of rat alpha IIb beta 3 and alpha v beta 3, blocks in vivo thrombus formation and in vitro angiogenesis", THROMB HAEM, 85(5), 2001, pp. 896-902

Abstract

Abciximab (c7E3 Fab. ReoPro (R)) blocks GPIIb/IIIa and alpha nu beta3 and inhibits thrombotic and proliferative events only in humans and nonhuman primates. The bivalent F(ab ')(2) fragment is an effective antithrombotic agent in canine models. In the present study, 7E3 F(ab ')(2) was also found tobind to rat GPIIb/IIla (K-D = 27 +/- 4 mug/mL) and alpha nu beta3 (K-D = 9+/- 8 mug/mL). to block in vitro rat platelet aggregation (IC50 = 16 +/- 6mug/mL). and to inhibit alpha nu beta3-mediated microvessel sprout formation in a rat aortic ring assay. Following administration of 7E3 F(ab ')(2) (4 mg/kg) to rats, platelet aggregation was completely blocked for up ro 6 hand thrombus formation in response to a rat abdominal aorta double crush injury was prevented. Effective chronic dosing was achieved with 6 mg/kg daily I.P. injections. In vitro mixing experiments indicated that 7E3 F(ab ')(2) redistributed to unlabeled platelets in 2 h, Ex vivo. 7E3 F(ab ')(2) wasdetected on platelets for up to 4 days after a single 4-mg/kg injection. These data suggest that 7E3 F(ab ')(2) may be a useful agent to study the effects of GPIIb/IIIa and alpha nu beta3 blockade in rat models of thrombosisand vascular disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:52:37