Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Serum-mediated relaxant response to toluene diisocyanate (TDI) in isolatedguinea-pig bronchi
Autore:
Boschetto, P; Jovine, L; Chitano, P; De Marzo, N; Plebani, M; Faggian, D; Fabbri, LM; Mapp, CE;
Indirizzi:
Univ Ferrara, Dept Clin & Expt Med, Ferrara, Italy Univ Ferrara Ferrara Italy errara, Dept Clin & Expt Med, Ferrara, Italy Duke Univ, Med Ctr, Dept Pediat, Durham, NC USA Duke Univ Durham NC USADuke Univ, Med Ctr, Dept Pediat, Durham, NC USA Univ Padua, Inst Clin Chem, Padua, Italy Univ Padua Padua ItalyUniv Padua, Inst Clin Chem, Padua, Italy Univ Modena, Dept Med Oncol & Radiol, I-41100 Modena, Italy Univ Modena Modena Italy I-41100 d Oncol & Radiol, I-41100 Modena, Italy Univ Padua, Dept Publ Hlth & Environm Med, Padua, Italy Univ Padua PaduaItaly dua, Dept Publ Hlth & Environm Med, Padua, Italy
Titolo Testata:
RESPIRATORY MEDICINE
fascicolo: 5, volume: 95, anno: 2001,
pagine: 357 - 362
SICI:
0954-6111(200105)95:5<357:SRRTTD>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE; OCCUPATIONAL ASTHMA; MECHANISMS; CAPSAICIN; PROTEINS; AIRWAYS;
Keywords:
bronchial smooth muscle; isocyanates; sensory nerves; serum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Mapp, CE Univ Ferrara, Sez Igiene & Med Lavoro, Via Fossato Mortara 64-B, I-44100 Ferrara, Italy Univ Ferrara Via Fossato Mortara 64-B Ferrara ItalyI-44100 taly
Citazione:
P. Boschetto et al., "Serum-mediated relaxant response to toluene diisocyanate (TDI) in isolatedguinea-pig bronchi", RESP MED, 95(5), 2001, pp. 357-362

Abstract

The present study was designed to evaluate whether pre-incubation with serum, obtained from both control and toluene diisocyanate (TDI)-immunized guinea-pigs, modified the contractile response to TDI in isolated guinea-pig bronchial rings. Guinea-pigs were anaesthetized and the main bronchi dissected in two rings. Bronchial rings were incubated with normal or immune serum (100 mul ml(-1) for 2h) and dose-response curves to TDI (0(.)03-1000 muM) were studied isometrically. Before serum incubation. in eight bronchial rings, epithelium was removed by rubbing the luminal surface gently with a gauze. In control rings. TDI produced a concentration-dependent contraction, whereas in rings pre-incubated with either normal or TDI-immune serum, it produced a concentration-dependent relaxation. Relaxation was 101(.)4 (SEM 17(.)4)% and 94(.)9 (SEM 21)% Of the relaxation induced by isoproterenol (1 mM) respectively with normal and TDI-immune serum. Similarly to the pre-incubation with serum, pre-incubation with albumin produced a concentration-dependent relaxation to TDI. Serum-induced relaxant response to TDI was not affected by capsaicin desensitization. it was only partially inhibited by an NK1-tachykinin antagonist, whereas it was blocked by indomethacin. In bronchial rings without epithelium, pre-incubated with serum. TDI caused contraction at highest doses. while it still induced relaxation at the lowest doses. This study shows that one or more components of the serum modify the contractile response to TDI in isolated guinea-pig bronchi. In bronchial rings without epithelium serum was able to inhibit the contration induced by low doses of TDI.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 23:25:30