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Titolo:
STABILIZATION OF ACETYLCHOLINE-RECEPTORS BY EXOGENOUS ATP AND ITS REVERSAL BY CAMP AND CALCIUM
Autore:
OMALLEY JP; MOORE CT; SALPETER MM;
Indirizzi:
CORNELL UNIV,NEUROBIOL & BEHAV SECT,SEELEY G MUDD HALL ITHACA NY 14853 CORNELL UNIV,NEUROBIOL & BEHAV SECT ITHACA NY 14853
Titolo Testata:
The Journal of cell biology
fascicolo: 1, volume: 138, anno: 1997,
pagine: 159 - 165
SICI:
0021-9525(1997)138:1<159:SOABEA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
VERTEBRATE NEUROMUSCULAR-JUNCTIONS; DEGRADATION RATE; METABOLIC STABILIZATION; POSTSYNAPTIC CHANGES; GENE-EXPRESSION; MUSCLE-ACTIVITY; MOUSE MUSCLE; INVIVO; SYNAPSES; ADULT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J.P. Omalley et al., "STABILIZATION OF ACETYLCHOLINE-RECEPTORS BY EXOGENOUS ATP AND ITS REVERSAL BY CAMP AND CALCIUM", The Journal of cell biology, 138(1), 1997, pp. 159-165

Abstract

Innervation of the neuromuscular junction (nmj) affects the stabilityof acetylcholine receptors (AChRs). A neural factor that could affectAChR stabilization was studied using cultured muscle cells since theyexpress two distinct populations of AChRs similar to those seen at the nmjs of denervated muscle, These two AChR populations are (in a ratio of 9 to 1) a rapidly degrading population (Rr) with a degradation half-life of similar to 1 d and a slowly degrading population (Rs) that can alternate between an accelerated form (half-life similar to 3-5 d)and a stabilized form (half-life similar to 10 d), depending upon thestale of innervation of the muscle. Previous studies have shown that elevation of intracellular cAMP can stabilize the Rs, but not the Rr. We report here that in cultured rat muscle cells, exogenous ATP stabilized the degradation half-life of Rr and possibly also the Rs. Furthermore, pretreatment with ATP caused more stable AChRs to be inserted into the muscle membrane. Thus, in the presence of ATP, the degradation rates of the Rr and Rs overlap, This suggests that ATP released from the nerve may play an important role in the regulation of AChR degradation. Treatment with either the cAMP analogue dibutyryl-cAMP (dB-cAMP) or the calcium mobilizer ryanodine caused the ATP-stabilized Rr to accelerate back to a half-life of 1 d. Thus, at least three signaling systems (intracellular cAMP, Ca2+, and extracellular ATP) have the potential to interact with each other in the building of an adult neuromuscular junction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 15:57:39