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Titolo:
Analysis of a donor gene region for a variant surface glycoprotein and itsexpression site in African trypanosomes
Autore:
LaCount, DJ; El-Sayed, NM; Kaul, S; Wanless, D; Turner, CMR; Donelson, JE;
Indirizzi:
Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 a, Dept Biochem, Iowa City, IA 52242 USA Inst Genom Res, Rockville, MD 20850 USA Inst Genom Res Rockville MD USA 20850 Genom Res, Rockville, MD 20850 USA Univ Glasgow, IBLS, Div Infect & Immun, Glasgow G12 8QQ, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland G12 8QQ ow G12 8QQ, Lanark, Scotland
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 10, volume: 29, anno: 2001,
pagine: 2012 - 2019
SICI:
0305-1048(20010515)29:10<2012:AOADGR>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIGENIC VARIATION; MOBILE ELEMENT; VSG GENE; BRUCEI-RHODESIENSE; POINT MUTATIONS; METACYCLIC VSG; MESSENGER-RNAS; TRANSCRIPTION; INFECTIONS; SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Donelson, JE Univ Iowa, Dept Biochem, 51 Newton Rd, Iowa City, IA 52242 USA Univ Iowa 51 Newton Rd Iowa City IA USA 52242 y, IA 52242 USA
Citazione:
D.J. LaCount et al., "Analysis of a donor gene region for a variant surface glycoprotein and itsexpression site in African trypanosomes", NUCL ACID R, 29(10), 2001, pp. 2012-2019

Abstract

African trypanosomes evade the immune response of their mammalian hosts bysequentially expressing genes for different variant surface glycoproteins (VSGs) from telomere-linked VSG expression sites. In the Trypanosoma broceiclone whose genome is being sequenced (GUTat 10.1), we show that the expressed VSG(VSG 10.1) is duplicated from a silent donor VSG located at anothertelomere-linked site. We have determined two 130 kb sequences representingthe VSG 10.1 donor and expression sites. The telomere-linked donor VSG 10.1 resembles metacyclic VSG expression sites, and is preceded by a cluster of 35 or more tandem housekeeping genes, all of which are transcribed away from the telomere. The 45 kb telomere-linked VSG 10.1 expression site contains a promoter followed by seven expression site-associated genes (ESAGs), three pseudo ESAGs, two pseudo VSGs and VSG 10.1. The 80 kb preceding the expression site has few, if any, functional ORFs, but contains 50 bp repeats,INGI retrotransposon-like elements, and novel 4-12 kb repeats found near other telomeres. This analysis provides the first look over a 130 kb range of a telomere-linked donor VSG and its corresponding telomere-linked VSG expression site and forms the basis for studies on antigenic variation in the context of a completely sequenced genome.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 00:27:19