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Titolo:
Protein kinase C modulates NMDA receptor trafficking and gating
Autore:
Lan, JY; Skeberdis, VA; Jover, T; Grooms, SY; Lin, Y; Araneda, RC; Zheng, X; Bennett, MVL; Zukin, RS;
Indirizzi:
Yeshiva Univ Albert Einstein Coll Med, Dept Neurosci, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA
Titolo Testata:
NATURE NEUROSCIENCE
fascicolo: 4, volume: 4, anno: 2001,
pagine: 382 - 390
SICI:
1097-6256(200104)4:4<382:PKCMNR>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
METHYL-D-ASPARTATE; LONG-TERM POTENTIATION; GLUTAMATE RECEPTORS; OPEN PROBABILITY; PYRAMIDAL CELLS; RAT HIPPOCAMPUS; XENOPUS OOCYTES; NR1 SUBUNIT; PHOSPHORYLATION; CHANNEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Bennett, MVL Yeshiva Univ Albert Einstein Coll Med, Dept Neurosci, 1300 Morris Pk Ave, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med 1300Morris Pk Ave Bronx NY USA 10461
Citazione:
J.Y. Lan et al., "Protein kinase C modulates NMDA receptor trafficking and gating", NAT NEUROSC, 4(4), 2001, pp. 382-390

Abstract

Regulation of neuronal N-methyl-D-aspartate receptors (NMDARs) by protein kinases is critical in synaptic transmission. However, the molecular mechanisms underlying protein kinase C (PKC) potentiation of NMDARs are uncertain. Here we demonstrate that PKC increases NMDA channel opening rate and delivers new NMDA channels to the plasma membrane through regulated exocytosis. PKC induced a rapid delivery of functional NMDARs to the cell surface and increased surface NR1 immunofluorescence in Xenopus oocytes expressing NMDARs. PKC potentiation was inhibited by botulinum neurotoxin A and a dominantnegative mutant of soluble NSF-associated protein (SNAP-25), suggesting that receptor trafficking occurs via SNARE-dependent exocytosis. In neurons, PKC induced a rapid delivery of functional NMDARs, assessed by electrophysiology, and an increase in NMDAR clusters on the surface of dendrites and dendritic spines, as indicated by immunofluorescence. Thus, PKC regulates NMDAR channel gating and trafficking in recombinant systems and in neurons, mechanisms that may be relevant to synaptic plasticity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:06:20