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Titolo:
Immunohistochemical detection of HCV in cirrhosis, dysplastic nodules, andhepatocellular carcinomas with parallel-tissue quantitative RT-PCR
Autore:
Rullier, A; Trimoulet, P; Urbaniak, R; Winnock, M; Zauli, D; Ballardini, G; Rosenbaum, J; Balabaud, C; Bioulac-Sage, P; Le Bail, B;
Indirizzi:
Univ Bordeaux 2, INSERM E9917, Lab Pathol, GREF, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 , GREF, F-33076 Bordeaux, France Hop Pellegrin, Virol Lab, F-33076 Bordeaux, France Hop Pellegrin Bordeaux France F-33076 irol Lab, F-33076 Bordeaux, France Hop Pellegrin, Serv Anat Pathol, F-33076 Bordeaux, France Hop Pellegrin Bordeaux France F-33076 t Pathol, F-33076 Bordeaux, France Univ Bologna, Dipartimento Med Interna, Bologna, Italy Univ Bologna Bologna Italy na, Dipartimento Med Interna, Bologna, Italy
Titolo Testata:
MODERN PATHOLOGY
fascicolo: 5, volume: 14, anno: 2001,
pagine: 496 - 505
SICI:
0893-3952(200105)14:5<496:IDOHIC>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATITIS-C VIRUS; IN-SITU HYBRIDIZATION; POLYMERASE CHAIN-REACTION; CORE PROTEIN; LIVER BIOPSIES; MONITOR TEST; VIRAL-RNA; ANTIGENS; CELLS; EXPRESSION;
Keywords:
dysplastic nodules; HCV; hepatocellular carcinoma; immunohistochemistry; in situ detection; liver neoplasms; quantitative RT-PCR; viral carcinogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Le Bail, B Univ Bordeaux 2, INSERM E9917, Lab Pathol, GREF, 146 Rue Leo Saignat, F-33076 Bordeaux, France Univ Bordeaux 2 146 Rue Leo Saignat Bordeaux France F-33076 ce
Citazione:
A. Rullier et al., "Immunohistochemical detection of HCV in cirrhosis, dysplastic nodules, andhepatocellular carcinomas with parallel-tissue quantitative RT-PCR", MOD PATHOL, 14(5), 2001, pp. 496-505

Abstract

Hepatitis C virus is a major risk factor for hepatocarcinogenesis in humans. In situ detection of the virus in early sequential lesions of hepatocarcinogenesis could provide information about the role of the virus in the transformation and promotion process. Parallel in situ detection of HCV proteins and RNA in human tissues were performed in 55 posthepatitis C cirrhosis,17 dysplastic nodules (DN), and 25 hepatocellular carcinomas (HCC), using inmnunohistochemistry and tissue quantitative RT-PCR A consistent cytoplasmic hepatocellular staining was obtained in 73% of cirrhosis cases (with or without HCC) and in 55% DN cases. A few tumoral hepatocytes were unambiguously stained in 28% HCC. The percentage of positive cells and the intensity of immunostaining significantly decreased from cirrhosis to HCC through DN,whereas there was no difference in the prevalence of positivity or the number of viral copies between cirrhosis and HCC using tissue-quantitative RT-PCR, Finally, RT-PCR levels were found parallel with the immunostaining in cirrhosis but not in HCC. These results suggest that HCV protein synthesis may persist but be down-regulated during sequential hepatocarcinogenesis. Aputative role of HCV proteins on cell proliferation and differentiation during the early steps of carcinogenesis cannot therefore be excluded.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 04:12:06