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Titolo:
Synthesis and structural organization of zonular fibers during developmentand aging
Autore:
Hanssen, E; Franc, S; Garrone, R;
Indirizzi:
UCBL, UMR 5086, CNRS, Inst Biol Chim Prot, F-69367 Lyon 07, France UCBL Lyon France 07 , CNRS, Inst Biol Chim Prot, F-69367 Lyon 07, France
Titolo Testata:
MATRIX BIOLOGY
fascicolo: 2, volume: 20, anno: 2001,
pagine: 77 - 85
SICI:
0945-053X(200104)20:2<77:SASOOZ>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBRILLIN-CONTAINING MICROFIBRILS; ELASTIN-ASSOCIATED MICROFIBRILS; MARFAN-SYNDROME; VITREOUS-HUMOR; IX COLLAGEN; EXPRESSION; IDENTIFICATION; EXTRACTION; COMPONENT; LINKAGE;
Keywords:
extracellular matrix; development; aging; ciliary epithelium; in situ hybridization; electron microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Franc, S UCBL, UMR 5086, CNRS, Inst Biol Chim Prot, 7,Passage Vercors, F-69367 Lyon07, France UCBL 7,Passage Vercors Lyon France 07 rs, F-69367 Lyon07, France
Citazione:
E. Hanssen et al., "Synthesis and structural organization of zonular fibers during developmentand aging", MATRIX BIOL, 20(2), 2001, pp. 77-85

Abstract

Zonular fibers are a specific form of extracellular matrix composed mainlyof fibrillins. The purpose of this study was to determine which cells secrete fibrillin-1 during development and aging. A specific guinea pig fibrillin-1 mRNA probe was designed and cloned in order to identify fibrillin-secreting cells in guinea pig eye, using in situ hybridization. Immunofluorescence, with a specific guinea pig monoclonal antibody, was used to compare protein levels at different stages from birth to 35 months of age. Electron microscopy and immunolabeling were used to investigate the organization of zonular microfibril bundles. We identified the cells of non-pigmented epithelium of the ciliary body as the main source of fibrillin secreted into the zonule. Moreover, while mRNA expression decreased during aging, there was no decrease in fibrillin immunoreactivity, as previously described in human aorta. These data indicate a very slow turnover of the zonular microfibrilswhich can be correlated with the appearance during aging of a new periodicfibrillar structure. This new structure may reflect an increased cross-linking in the long-lived zonular microfibrillar bundles. (C) 2001 Elsevier Science B.V./International Society of Matrix Biology. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 11:06:16