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Titolo:
GENERALIZED TRANSDUCTION IN THE POTATO BLACKLEG PATHOGEN ERWINIA-CAROTOVORA SUBSP ATROSEPTICA BY BACTERIOPHAGE-PHI-M1
Autore:
TOTH IK; MULHOLLAND V; COOPER V; BENTLEY S; SHIH YL; PEROMBELON MCM; SALMOND GPC;
Indirizzi:
UNIV CAMBRIDGE,DEPT BIOCHEM,TENNIS COURT RD CAMBRIDGE CB2 1QW ENGLAND UNIV CAMBRIDGE,DEPT BIOCHEM CAMBRIDGE CB2 1QW ENGLAND UNIV WARWICK,DEPT BIOL SCI COVENTRY CV4 7AL W MIDLANDS ENGLAND SCOTTISH CROP RES INST DUNDEE DD2 5DA SCOTLAND
Titolo Testata:
Microbiology
, volume: 143, anno: 1997,
parte:, 7
pagine: 2433 - 2438
SICI:
1350-0872(1997)143:<2433:GTITPB>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOFT-ROT ERWINIAS; DISCRIMINATION; RECOMBINATION; CHRYSANTHEMI; ENZYMES; PHAGE;
Keywords:
ERWINIA CAROTOVORA SUBSP ATROSEPTICA; GENERALIZED TRANSDUCTION; BACTERIOPHAGE; PHI-M1; POTATO BLACKLEG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
I.K. Toth et al., "GENERALIZED TRANSDUCTION IN THE POTATO BLACKLEG PATHOGEN ERWINIA-CAROTOVORA SUBSP ATROSEPTICA BY BACTERIOPHAGE-PHI-M1", Microbiology, 143, 1997, pp. 2433-2438

Abstract

Using enrichment methods, a new bacteriophage (phi M1) was isolated, which is capable of generalized transduction in Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043. phi M1 is probably a virulent phage and contains double-stranded DNA of approximately 43 kb. Transduction frequencies for a number of chromosomal markers and plasmid pHCP2were established, and conditions for transduction optimized. UV irradiation of the lysates prior to transduction enhanced the transduction frequency. phi M1 infected over 25% of Eca strains tested and so may be useful both for the genetic analysis of a number of Eca isolates andfor the transductional transfer of selectable markers between strains.

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Documento generato il 27/10/20 alle ore 04:32:41