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Titolo:
Immunohistochemical localization of trichloroacylated protein adducts in tetrachloroethene-treated mice
Autore:
Green, SM; Khan, MF; Kaphalia, BS; Ansari, GAS;
Indirizzi:
Univ Texas, Med Branch, Dept Pathol, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 h, Dept Pathol, Galveston, TX 77555 USA Univ Texas, Med Branch, Dept Pharmacol & Toxicol, Galveston, TX 77550 USA Univ Texas Galveston TX USA 77550 acol & Toxicol, Galveston, TX 77550 USA
Titolo Testata:
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
fascicolo: 2, volume: 63, anno: 2001,
pagine: 145 - 157
SICI:
1528-7394(20010525)63:2<145:ILOTPA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
VINYL-CHLORIDE DISEASE; MRL-+/+ MICE; IMMUNOCHEMICAL DETECTION; GLUTATHIONE CONJUGATION; AUTOIMMUNE-RESPONSE; TRICHLOROETHYLENE; RATS; PERCHLOROETHYLENE; CARCINOGENICITY; EXPOSURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Ansari, GAS Univ Texas, Med Branch, Dept Pathol, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 ol, Galveston, TX 77555 USA
Citazione:
S.M. Green et al., "Immunohistochemical localization of trichloroacylated protein adducts in tetrachloroethene-treated mice", J TOX E H A, 63(2), 2001, pp. 145-157

Abstract

Tetrachloroethene (PCE), a common industrial solvent and environmental contaminant is primarily used in the dry-cleaning industry. The toxicity of PCE has bt en linked to vision disorders, renal and hepatic cancer, and autoimmune diseases. Although the mechanism of toxicity is not fully understood. PCE forms trichloroacylated protein adducts in tissues where toxicity is known to occur. These adducts may be responsible for toxicity by altering the function of cellular proteins. Using Western blot analysis, formation of trichloroacylated protein adducts has been reported. To determine the localization of the adducts in a specific zone of a tissue, immunohistochemical staining was used in the study. An antiserum to trichloroacylated proteins was raised in rabbits and its specificity was established by enzyme-linked immunosorbent assay (ELISA). Female MRL-lpr/lpr and MRL +/+ mice were treated with PCE using a single 5-mmol/kg dose over 24 h or on every fourth day for 6 wk (total 20 doses). Formation of trichloroacylated protein adducts was observed in the liver, and localized to the centrilobular zones. Intensity and circumference of the staining around the central vein were much greater in subchronically treated mice than in acutely treated mice. No immunochemical reactivity was observed in any of the other tissues examined. This study shows that hepatic trichloroacylated protein adducts are localized ina region of the liver where PCE-mediated toxicity is known to occur. Immunohistochemical localization of there adducts and its association with PCE-induced toxicity support the contention that adducts may contribute to toxicity.

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Documento generato il 05/04/20 alle ore 02:38:35