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Titolo:
Inhibition of calcium-stimulated ATPase in the hen brain P2 synaptosomal fraction by organophosphorus esters: Relevance to delayed neuropathy
Autore:
Barber, D; Hunt, J; Ehrich, M;
Indirizzi:
Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA Virginia Polytech Inst& State Univ Blacksburg VA USA 24061 VA 24061 USA
Titolo Testata:
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
fascicolo: 2, volume: 63, anno: 2001,
pagine: 101 - 113
SICI:
1528-7394(20010525)63:2<101:IOCAIT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SARCOPLASMIC-RETICULUM; PLASMA-MEMBRANE; TARGET ESTERASE; NEUROTOXICITY; CHLORPYRIFOS; ACETYLCHOLINESTERASE; METHYLPARATHION; INSECTICIDES; PARATHION; FLUIDITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Ehrich, M Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA Virginia Polytech Inst & State Univ Blacksburg VA USA 24061 USA
Citazione:
D. Barber et al., "Inhibition of calcium-stimulated ATPase in the hen brain P2 synaptosomal fraction by organophosphorus esters: Relevance to delayed neuropathy", J TOX E H A, 63(2), 2001, pp. 101-113

Abstract

Organophosphorus (OP) compounds have been reported to inhibit Ca/Mg-ATPase. bur the relevance of this inhibition to organophosphate-induced delayed neuropathy (OPIDN) has nor been explored. To determine if inhibition of thisenzyme was related to the development of OPIDN, neuropathic and nonneuropathic OP compounds were tested for their ability to inhibit Ca-stimulated ATPase activity in the P2 synaptosomal fraction from hen brain. Following in vitro exposure to 10(-3) to 10(-5) M OP compounds, Ca-stimulated ATPase activity was inhibited by chlorpyrifos, chlorpyrifos-oxon, phenyl saligenin phosphate (PSP), and tri-o-tolyl phosphate (TOTP), but nor by parathion, paraoxon, or diisopropyl fluorophosphate (DFP). Further investigation of inhibition induced by chlorpyrifos determined that inhibition was noncompetitive with respect to calcium and ATP. OP compound hydrophobicity was well correlated with in vitro inhibition of Ca-stimulated ATPase, suggesting that OP compounds interact with membrane lipids, and this interaction may contributeto the noncompetitive inhibition of Ca-stimulated ATPase that was observed. Subsequent to in vivo exposure, DFP, but not PSP, produced inhibition of Ca-stimulated ATPase activity in the hen brain P2 synaptosomal Fraction. These data indicate that inhibition of Ca-stimulated ATPase activity is not correlated to neuropathic potential and demonstrate that inhibition of Ca/Mg-ATPase is not responsible for OPIDN.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 15:45:02