Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of rat prostatic steroid binding protein (PSBP) as an immunosuppressive factor
Autore:
Maccioni, M; Riera, CM; Rivero, VE;
Indirizzi:
Univ Nacl Cordoba, Fac Ciencias Quim, Dept Bioquim Clin, RA-5000 Cordoba, Argentina Univ Nacl Cordoba Cordoba Argentina RA-5000 , RA-5000 Cordoba, Argentina
Titolo Testata:
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
fascicolo: 2, volume: 50, anno: 2001,
pagine: 133 - 149
SICI:
0165-0378(200105)50:2<133:IORPSB>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SEMINAL PLASMA; EXTRACELLULAR ORGANELLES PROSTASOMES; BLOOD MONONUCLEAR-CELLS; SERUM-FREE MEDIUM; MAJOR PROTEIN; VESICLE EPITHELIUM; SV-IV; ESTRAMUSTINE; UTEROGLOBIN; EXPRESSION;
Keywords:
prostatein; immunosuppression; rat; proliferation; IL-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Rivero, VE Univ Nacl Cordoba, Fac Ciencias Quim, Dept Bioquim Clin, RA-5000 Cordoba, Argentina Univ Nacl Cordoba Cordoba Argentina RA-5000 ordoba, Argentina
Citazione:
M. Maccioni et al., "Identification of rat prostatic steroid binding protein (PSBP) as an immunosuppressive factor", J REPRO IMM, 50(2), 2001, pp. 133-149

Abstract

Prostatic steroid binding protein (PSBP) is the major protein produced ( similar to 20% of the total cytosolic protein) and secreted into the seminalfluid by the rat ventral prostate but its physiological function has not been elucidated yet. Since PSBP is secreted into the seminal fluid (which isitself a potent immunosuppressor) and has strong homology with uteroglobin(which possess an important anti-inflammatory function) our aim was to determine what effect, if any, PSBP would have on the immune system. With thatpurpose in mind we performed mononuclear cell cultures in the presence or absence of purified PSBP and analysed the effect of this protein on different functional parameters. PSBP inhibits the mitogen-induced proliferation of normal rat spleen mononuclear cells (MNC) specifically and in a dose-dependent manner. It reduces the production of IL-2 and the expression of its receptor (analysed by flow cytometry) which are important events for lymphocyte proliferation. Also, PSBP was able to inhibit OVA-specific proliferation of lymph node cells from previously primed animals. The immunosuppressiveeffect of PSBP is not due to an inherent toxic effect to the cells, since the cell viability was kept intact at the different times of culture studied. We also analysed the effect of rat PSBP on mitogen-induced proliferationof mouse spleen and human blood MNC. The proliferation was strongly abolished in a dose-dependent and non-species specific fashion. Moreover, PSBP strongly inhibits the human mixed lymphocyte reaction. Taken together, the present data support evidence for a new type of function for PSBP. We report that PSBP is a potent immunosuppressor factor and we describe its effect onthe immune function in vitro. Here, we discuss the possible implications of these findings in the protection of sperm from immunologic damage in the feminine reproductive tract. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:18:34