Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The use of impedance spectroscopy for the characterization of protein-modified ISFET devices: Application of the method for the analysis of biorecognition processes
Autore:
Kharitonov, AB; Wasserman, J; Katz, E; Willner, I;
Indirizzi:
Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91904 -91904 Jerusalem, Israel
Titolo Testata:
JOURNAL OF PHYSICAL CHEMISTRY B
fascicolo: 19, volume: 105, anno: 2001,
pagine: 4205 - 4213
SICI:
1520-6106(20010517)105:19<4205:TUOISF>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIELD-EFFECT TRANSISTORS; ION-SELECTIVE ELECTRODES; BIOSENSOR; MEMBRANE; IMMUNOSENSOR; INTEGRATION; SENSORS; SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Willner, I Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91904 salem, Israel
Citazione:
A.B. Kharitonov et al., "The use of impedance spectroscopy for the characterization of protein-modified ISFET devices: Application of the method for the analysis of biorecognition processes", J PHYS CH B, 105(19), 2001, pp. 4205-4213

Abstract

Impedance spectroscopy is used to characterize the structure of biomaterial layers on the gate surface of ISFET devices, and to elucidate antigen-antibody binding interactions on the gate interface. The method is based on the recording of the transfer function of the system as a function of frequency and on the extraction of the respective iota (1) and iota (2) values of the systems. From the experimental values, the film thicknesses of the respective protein layers are estimated. The method is applied to elucidate thestructures of a glucose oxidase, GOx, multilayer assembly, and of a di-biotin-cross-linked avidin multilayer system. The method is also used to sensethe dinitrophenyl antibody by following the formation of the antigen-antibody complex on the gate surface.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 22:10:05